FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL16_Col_B_j1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL16_Col_B_j1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1033644
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT23610.22841519904338436TruSeq Adapter, Index 19 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGCG305.879347E-446.66631356
GCCGTCT3750.045.7329951
TGCCGTC4200.041.6663550
TATGCCG4250.040.35263448
ATGCCGT4500.038.8885949
CGGACTG2200.035.0014276
CGTATGC5150.034.6599346
GTATGCC5200.034.32666447
CCGTCTT5000.033.59974352
GCGGACT1055.511538E-1033.3346945
CTGCTTG5400.029.81458959
CTCGTAT5550.029.63941444
TCGTATG5800.029.56874345
TCTCGTA5600.028.7497843
GGACTGT2750.028.001147
CGGACTC1502.910383E-1128.001146
CGGACTA1154.9016307E-827.3924226
GACTGTA2450.027.1439658
TCGGACT1850.026.4875685
GCTATCT6600.024.39375339
Produced by FastQC (version 0.11.5)