FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL15_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL15_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6350215
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT70110.11040571067278825TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC14500.038.62027750
TATGCCG15750.038.22181348
ATGCCGT15150.036.5012649
CGTATGC17750.033.5207746
GCCGTCT15200.033.15806251
GTATGCC18400.032.90725747
CTCGTAT23700.024.81005744
TCGTATG24950.023.56706845
TCGTATC31000.022.241738
CGTATCT29650.021.12979139
CCGTCTT24900.020.52219252
TATCTCG30700.020.40710641
TCTCGTA31750.018.18892543
CTGCTTG25150.017.95209559
TTCGTAT42600.017.17117937
GTATCTC37600.016.9414440
ATCTCGT36700.016.5939642
CACGAAT48950.015.44416631
ATTCGTA49700.015.42237136
GAATTCG50550.015.02456834
Produced by FastQC (version 0.11.5)