FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL14_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL14_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12010363
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT145940.12151173116083169TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG31750.037.25957548
TGCCGTC32350.034.40469450
GTATGCC38200.032.4343247
GCCGTCT31150.031.01113751
ATGCCGT37750.030.78123549
CGTATGC47950.025.32828346
CTCGTAT51650.022.29412744
CGTATCT57000.022.10510439
TCGTATC63000.021.94428638
TCGTATG58300.019.8712145
TCTCGTA66700.017.26374243
TTCGTAT88450.016.8964337
CTGCTTG51550.016.7021159
CCGTCTT63150.016.57160452
ATCTCGT72750.016.40537842
TATCTCG76400.016.30878341
GAATTCG98150.015.83279434
CACGAAT99250.015.48099631
CTGCGTG11700.014.95715659
CGAATTC104250.014.83922133
Produced by FastQC (version 0.11.5)