FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL13_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL13_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8053321
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG18650.033.40463348
TGCCGTC18450.031.68003750
GCCGTCT17100.030.70157851
ATGCCGT19800.029.52003549
CGTATGC21700.028.38693246
GTATGCC22850.027.41778447
CGGACTG10600.025.0948186
CTCGTAT28300.021.5193144
TCGTATG31200.019.40693945
CCGTCTT29050.018.67459152
TCGTATC41400.018.26076538
CTGCGTG7000.017.499959
CGTATCT38500.017.36353739
TATCTCG39050.016.49158341
TCTCGTA37150.016.39290843
CTGCTTG32050.015.5069359
GGACTGT16950.014.867547
ATTCGTA58650.014.8592536
TTCGTAT57750.014.30294837
GTATCTC47150.014.02961240
Produced by FastQC (version 0.11.5)