FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL13_C24_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL13_C24_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1288734
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG4200.053.333256
GGACTGT4250.049.4116827
GACTGTA5050.036.7326138
TGCCGTC2400.030.62494950
CAGTCCG4400.030.22722227
GCCGTCT2100.029.9999551
CTGTAGA6600.029.16661810
TCGGACT3550.028.5915035
TATGCCG2600.028.26918448
ACTGTAG6600.028.1060149
CCGGACT2500.027.9999525
ACGGACT5150.026.504815
AACGGAC2250.026.44444
ACAGTCC5300.025.75467526
TACAGTC5700.023.33329225
CTACAGT6250.022.39996324
GTATGCC3300.021.21208647
GTAGAAG9000.020.99996612
GCGGACT1506.38669E-720.9999665
TCTACAG6800.020.58820223
Produced by FastQC (version 0.11.5)