FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL12_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL12_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7633619
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14800.029.3241348
TGCCGTC15150.027.49156650
GTATGCC18300.024.67196847
GCCGTCT15250.024.09820251
ATGCCGT17900.024.0501249
TCGTATC34700.016.23908638
CGTATCT31550.015.97453839
CGTATGC29950.015.42560846
CTCGTAT29550.015.27908744
ATCTCGT38500.013.36354742
TCGTATG34050.013.36261245
GAATTCG48200.012.34431634
CCGTCTT34750.012.28768852
TCTCGTA39250.012.038136543
CGAATTC50600.011.96632433
CACGAAT49750.011.88936831
TTCGTAT51650.011.65529537
GTCACGA50200.011.36446629
CTGCTTG31250.011.31192559
GTCCCCC6501.8189894E-1211.30761769
Produced by FastQC (version 0.11.5)