FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL11_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL11_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3278602
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG6300.027.22217448
ATGCCGT6400.025.70308149
TGCCGTC6250.025.19995750
CGTATGC7000.024.99995846
CGGACTG4350.024.1378886
GTATGCC7400.023.64860547
GCCGTCT6150.022.76418951
CTCGTAT10100.018.36630444
TCGTATG10450.017.0813145
GGACTGT6850.016.3503367
CCGTCTT10000.015.39997452
TATCTCG12200.014.91800741
TCGTATC13450.014.83268838
ACTGTAG8600.014.6511379
GACTGTA6700.014.6268418
CGTATCT13350.014.15727939
TCTCGTA13400.014.10445343
AGGGGGG4103.266905E-912.8062231
GTATCTC15800.012.6265640
CTGCTTG10550.012.60661359
Produced by FastQC (version 0.11.5)