FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_ColSPL10_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_ColSPL10_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2525479
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT30170.11946248612639424TruSeq Adapter, Index 13 (97% over 38bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT6700.038.6565249
TATGCCG7200.034.99982548
TGCCGTC7200.034.99982550
CGTATGC8000.033.24983246
GCCGTCT7250.031.86190851
GTATGCC8500.031.29395747
TCGTATG10650.024.31912445
CCGTCTT10000.024.14987652
CTCGTAT10500.022.3332244
CTGCTTG10350.019.95158859
CGGACTG2900.019.3110126
TCTCGTA13550.017.56448643
CGTCTTC14400.017.01380353
AATCTCG15300.016.6992641
ATCTCGT15550.015.75554642
CTTCTGC14800.015.37154456
GGACTGT3704.3655746E-1115.1356577
CATTCAG22900.015.13092833
GCGGACT1951.4149929E-414.359475
GCTTGAA17800.013.96060461
Produced by FastQC (version 0.11.5)