FastQCFastQC Report
Fri 30 Aug 2019
HNLF5AFXY_n01_C24SPL11_Col_B_j.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNLF5AFXY_n01_C24SPL11_Col_B_j.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515808
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT5180.1004249643278119TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACTG2150.063.4882856
GGACTGT2550.052.156797
GACTGTA2550.046.6666038
CGGACTA1000.045.4999356
ACTGTAG3000.041.9999439
GCGGACT757.548806E-1041.9999435
ATCGGAC855.638867E-1141.176414
TCGGACT1550.040.6451075
AACGGAC1300.037.6922574
CTGTAGA3350.037.6118910
CCCGGAC601.21526045E-534.999954
TAGTCCG1300.034.9999511
CAGTCCG1850.034.05400527
ACGGACT2700.033.7036555
CTACAGT2200.031.81813824
GACTGTC450.00430631131.111078
CCGGACT1151.4570105E-930.4347445
CTAGTCC1500.030.33329210
ACAGTCC2200.028.63632426
TACAGTC2450.028.5713925
Produced by FastQC (version 0.11.5)