FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12743968
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4828763.7890553397497544No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2012051.5788253705596247No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG165650.12998306335985776No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA145530.11419520199674074No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA144570.11344190443667154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC27450.064.3035352
GCACGCA11350.059.7652782
GCAGGTC51400.058.7875069
CTATTCG58050.055.306349
GCAAAAG70300.054.4550133
CGCACTT13450.053.5953255
ACGCACT14050.049.7247624
CACGCAC14450.048.948323
GAGCAAA80650.048.7651181
GAGCGAA9600.048.6444851
TAAACTA80000.042.414075
TTTAAAC87050.037.9457633
CACGAGC14750.036.957878
AAACACG21000.034.673265
TTTTTAA103450.031.0346681
CAAAAGC130150.030.6417964
CAGGTCA51650.028.2854738
AGGTCGT34700.027.4761816
GGTCGTT36850.026.8303597
TAACAAG74500.026.7798585
Produced by FastQC (version 0.11.5)