FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_MICE_Exp2_Mother_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_MICE_Exp2_Mother_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883508
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA14830.16785360177836534No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG14020.15868560329957399No Hit
TACATACACTCATACACAATAGCTCCCAAAAAAGACCTAAGTACTCGGCG9760.1104687224111157No Hit
ATAATACACAATGCCATCTACCAAATATTACCCTTCCCCAAAGCAGCACA9570.10831820424942389No Hit
GTGTATTATATGGCATGAGTAGGGTAGCAGTGCATCTTGAAGTATGTAAA9380.1061676860877321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2350.067.887421
GCAAAAG19600.061.40283
GAGCAAA20650.058.644991
AGCGAAA4650.053.0225261
AGCAAAA24900.048.627042
CGAAAGC6250.039.439833
CAAAAGC31750.038.129384
AAAAGCA38900.031.8754585
CGAGACT1851.6449485E-731.347674
AAGCAGG44550.030.4354137
TTAGTAG1204.052389E-430.2150441
TTATAGT2901.2732926E-1129.9964754
GTACTAT2451.9663275E-929.5984121
AGCAGGC16950.029.0887138
AGCAGGG19050.028.9270348
GCGAAAG9000.028.195922
ACTATAG2705.5624696E-926.8517363
GTAATAG1701.02960345E-425.5939221
GAGACGG2301.1018255E-625.2201399
GCGTAGA1450.001224742725.0055541
Produced by FastQC (version 0.11.5)