FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_MICE_Exp1_Mother_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_MICE_Exp1_Mother_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960657
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG14770.15374894473261527No Hit
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA13900.14469264263936035No Hit
ATAATACACAATGCCATCTACCAAATATTACCCTTCCCCAAAGCAGCACA13860.14427626093392334No Hit
TACATACACTCATACACAATAGCTCCCAAAAAAGACCTAAGTACTCGGCG11130.11585820953784753No Hit
GTGTATTATATGGCATGAGTAGGGTAGCAGTGCATCTTGAAGTATGTAAA11110.11565001868512903No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2950.066.393091
GCAAAAG22000.059.659383
GAGCAAA24450.055.7776341
AGCAAAA26850.050.241122
TCCGCGC803.7359765E-545.30956145
AGCGAAA4950.041.0330661
AGCAGGC17300.034.7864078
CAAAAGC37450.034.267034
CGAAAGC6050.032.361693
AAGCAGG45750.032.330827
CTTAGAC7550.029.7740253
TAAGCGT1050.00770487227.6215696
GTACTAT3700.027.4477921
AAAAGCA47700.026.603735
TGTATTA9150.025.3641052
TATATGG8150.024.9102467
CAATTCG1500.001494795824.165098145
ATATGGC9100.023.9032788
TAGACTG8250.023.7319095
CCTACTT8300.023.582804145
Produced by FastQC (version 0.11.5)