FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_Exp2_Shed_Ind2_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_Exp2_Shed_Ind2_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1735103
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA42170.24304032671259285No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA41620.2398704860748901No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT32260.18592556176780284No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT26070.1502504462271116No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG24940.14373786455328588No Hit
TCTCTGTACTGAGAACGTAGGTTGTATGCTGATTTGGCCCGCAGATGCCC24640.14200886056908438No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG23950.13803215140542088No Hit
CTCAATATGAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAGC23440.1350928446322783No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC22390.129041330687573No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG22160.12771576096635184No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC22150.1276581275002118No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC21930.1263901912451307No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT21710.1251222549900496No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT21430.12350851793812817No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA21070.12143371315708636No Hit
CATATGGAGCATGCCCCAAGTATGTTAAGCAAAACACCCTGAAGTTGGCA20020.11538219921238106No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA19950.1149787649494007No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT19650.11324976096519918No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC19420.11192419124397802No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT19380.11169365737941782No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT19170.11048335459047676No Hit
CCTGTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGAT18900.1089272510046954No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG18700.10777458168189438No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT18160.10466237451033167No Hit
CCCTAGGAGGTCTGGAGCCGCAGGTGCTGCAGTCAAAGGAGTTGGAACAA17720.10212650200016944No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA17540.10108909960964854No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTACCG404.818503E-9108.74561145
GAGCGAA1950.0107.872121
AGCAGGC3600.080.559278
GTTGACG651.5661499E-978.07378145
AGCGAAA5000.072.53471
CTACTAA502.3059292E-672.505446
AGAGTTA300.001936292672.49708145
TATACGG2250.064.454852
GCTCACG451.19434335E-464.44184145
GGCGACC350.00356751562.140354145
GCAGGGT13450.061.4526449
TACGGGA2400.060.415974
ATATACG2500.058.027761
ATACGGG2500.058.0043453
TTATATA2650.057.4521684
CGAAAGC6700.055.19073
ACAAGGG7500.055.104133
GCAAAAG31600.054.6085243
AGCTTAT2700.053.729411
TATATCG1500.053.170655
Produced by FastQC (version 0.11.5)