FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_Exp2_Shed_Ind2_D1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_Exp2_Shed_Ind2_D1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296684
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT28340.21855748971993175No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG26500.20436744804439633No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC24860.1917198022031582No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT24690.19040876574400548No Hit
GTCTAGTAGAATAAGCATCTATTGGACAATAGTTAAGCCGGGAGACGTAC23980.18493326053224995No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG23050.17776111990276736No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC22660.17475344802588758No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT21000.16195156260121973No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA20700.15963796884977374No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT18850.14537080738252342No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT18520.1428258542559328No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA18280.14097497925477603No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA18270.14089785946306116No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA17800.13727322925246244No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT17310.13349435945843396No Hit
CTCCATATGTGATCTTGTTTACGTTTTGAAAGGGCTTGTCATTGGGAATG17040.1314121250821326No Hit
TGATAACACAGTTCGAGTCTCTGAAACTCTACAGAGATTCGCTTGGAGAA16690.12871293237211226No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA16590.1279417344549636No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT15800.12184927090948913No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT15530.11976703653318772No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT15420.1189187188243242No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT15140.11675936465630793No Hit
CATATGGAGCATGCCCCAAGTATGTTAAGCAAAACACCCTGAAGTTGGCA15100.11645088548944847No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT15030.1159110469474444No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA14850.1145228906965768No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA14580.1124406563202754No Hit
GAGTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAGGCAATG14390.11097538027769295No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC14320.11043554173568888No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA13870.1069651511085199No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC13320.10272356256420222No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA13300.1025693229807725No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA12970.10002436985418191No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2450.082.896061
GAGCGAA1150.081.995011
GGCAGCC1650.070.30078145
AAGGTCG1150.069.345619
ACAAGGG5550.064.01193
CAAGGTC1250.063.7979668
GCAGGGT8600.063.2247289
CGTATAT350.003566103862.1456643
GTGTTTA350.003567193462.140873145
TAAACTA2100.062.1408735
AAGGGTG5250.060.7599685
CAAGGGT6050.058.7193954
CGAAAGC3550.057.185683
TTTAAAC2300.056.7416953
ACAAGGT4000.056.185717
CTACTAA651.0940335E-555.767456
CAAGTTA553.2198662E-452.725586145
TACTGAT4300.050.579787
CCCGTAT450.00962191348.3560331
CCGTATA450.00963218248.3429762
Produced by FastQC (version 0.11.5)