FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_Exp2_Shed_Ind1_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_Exp2_Shed_Ind1_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1281376
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG118890.9278307069899858No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA111780.8723434807581849No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA100420.7836887845566017No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA60500.4721486901580801No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC55940.43656194590814873No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA53450.4171297105611467No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC50950.39761943410833356No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA44710.3489217840821117No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT44570.34782920860075417No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT41140.32106110930749443No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC38620.3013947506430587No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG37050.289142297030692No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT34260.26736882850935245No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA32200.2512923607122344No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG29270.22842631670953725No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT27330.21328634218215417No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG25160.19635142222111232No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG24220.18901555827485453No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC23060.1799627900007492No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT22080.17231476163124643No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC21740.1696613640336638No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT21720.1695052818220413No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA21280.16607147316634618No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA19620.1531166496016782No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCT19590.15288252628424442No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA19330.15085345753315188No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA19270.15038521089828435No Hit
AGTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATC19120.14921459431111556No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG19010.14835614214719178No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG19000.1482781010413805No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC18690.14585882676123166No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC18410.14367367579851661No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT18080.14109831930674524No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA17880.1395374971905202No Hit
CAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCG17800.13891316834403017No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA16910.13196750992682865No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT16730.1305627700222261No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC16540.1290799890118123No Hit
CTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGG16230.12666071473166346No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT15810.12338298828759084No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC15670.1222904128062333No Hit
CTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAA15640.12205628948879954No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT15300.11940289189121694No Hit
GAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAA14830.11573495991808805No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT14680.11456434333091926No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC14660.11440826111929676No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA14240.11113053467522414No Hit
GTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAA14080.1098818769822441No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGAT13940.10878930150088655No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT13780.10754064380790651No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT13760.10738456159628398No Hit
CTGTCTGGCTGTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACC13690.10683827385560524No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGTCTGCTGTAATTGGATATTTC13300.10379467072896635No Hit
TCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGTATGCTAGAGTCC13270.1035605474115326No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT13260.10348250630572137No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG13150.10262405414179757No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG13110.10231188971855254No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA13100.1022338486127413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2700.0134.341191
CTACTAA700.0113.935816
AGCGAAA7750.089.861251
GGCCGCC259.387898E-486.99873145
GCTCACG259.387898E-486.99873145
GAGCAAA43500.077.7140661
ATTAGTA755.4569682E-1177.380531
AGCAGGC4350.076.668558
GTCTGCG950.076.31468145
AGCTTAT1850.074.50491
GGCGACC300.001935978872.49894145
TAAACTA25200.071.0660255
GCAAAAG46650.071.033633
TTTAAAC25150.070.924553
CAAAAGC48200.068.889044
TTAGTAG851.6916601E-1068.255612
TTTTAAA26700.067.9041062
CGAAAGC10400.067.629773
TTTTTAA27650.065.591541
CGTTGGA350.00356583562.1468055
Produced by FastQC (version 0.11.5)