FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_Exp2_Shed_Cnt5_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_Exp2_Shed_Cnt5_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840399
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA2250.074.149961
GCGAAAG2850.058.522032
CGAAAGC2850.055.9642683
ATACGCG553.217943E-452.72985
TACGCGT700.001056295141.430566
TCGTTCA700.001056604441.428097145
CATCCCG3250.035.694025
TGCGACC850.002741092134.1192869
CGCGTGT850.002741092134.1192868
GTATACG850.00274189234.1172523
ATCCCGT3850.030.1313196
ATTAGAC4850.026.9069083
TCCCGTC4350.026.6679487
TCAGCCG1100.0096684626.36495
GCGTGTA1100.0096684626.36499
CCGTCAG4700.026.2246639
GTATAGT4150.026.2185571
CCCGTCA4850.025.4135918
AATTCGG2552.7064998E-622.744837145
GTGTATA5750.022.7075541
Produced by FastQC (version 0.11.5)