FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_Exp2_NL_Cnt5_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_Exp2_NL_Cnt5_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827989
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC169560.9275766976715943No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT132800.7264813956757945No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA106380.5819509854818601No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA98480.5387340952270501No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA93070.5091387311411611No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA79240.4334818207330569No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG68240.37330640392256187No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA56340.30820754391848093No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT54900.3003300348087434No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC52840.28906082038786884No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA52170.285395590454866No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA48200.2636777354787146No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC47580.26028603016757756No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG40990.22423548500565377No Hit
AATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCA40030.21898381226582875No Hit
GAGTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAGGCAATG33190.18156564399457548No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA31500.1723205117755085No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG30680.16783470797690794No Hit
GCCTAATACAGTGCTTAACATATTGAACATGCCCATCATCATTCCAGGGC30340.16597474054821992No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA29530.16154364167399257No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG28430.15552609999294306No Hit
ATAAGAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGA27950.15290026362303055No Hit
AGTCAGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCA27870.15246262422804513No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT27840.15229850945492562No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT27780.15197027990868653No Hit
CATATATGACAAGAAATCAGCCCGAATGGTTCAGAAATGTTCTAAGTATT27120.14835975490005684No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA27090.14819564012693728No Hit
TCGTAGAACTTGGTGATCCAAATGCACTTTTGAAGCACAGATTTGAAATA27060.14803152535381778No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA26720.14617155792512976No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG26220.14343631170647086No Hit
GAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAAGGCGA26120.14288926246273911No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA25960.14201398367276827No Hit
CCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAAAT25170.13769229464728724No Hit
ATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGC25150.13758288479854092No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA24950.13648878631107736No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA24740.13533998289924062No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG24590.13451940903364298No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA24260.13271414652932814No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA24080.13172945789061094No Hit
CTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAAG24020.13140122834437187No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC23320.12757188363824945No Hit
ATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTGAA23280.12735306394075677No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG22780.12461781772209789No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA22260.12177316165469267No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC22110.12095258778909501No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT21530.11777970217545072No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA21460.11739676770483849No Hit
GTTCAGAAATGTTCTAAGTATTGCTCCAATAATGTTCTCAAACAAAATGG21450.1173420627804653No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT21440.11728735785609212No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG21250.11624796429300177No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC20640.11291096390623795No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTT20630.11285625898186477No Hit
CAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCA20580.11258273435999888No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA20410.11165275064565487No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC20300.11105099647754993No Hit
ATATTGAACATGCCCATCATCATTCCAGGGCTCAATGATGCAGTCCCCTC20040.1096286684438473No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT19210.10508815972087358No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC18860.10317348736781239No Hit
ACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAG18760.10262643812408061No Hit
GTCTAGTAGAATAAGCATCTATTGGACAATAGTTAAGCCGGGAGACGTAC18690.10224350365346839No Hit
GTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAGGCAATGAG18450.10093058546851211No Hit
CTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAAATCC18430.10082117561976577No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT18320.10021942145166081No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCCG100.00709121145.00089145
ACGTGCG256.4661235E-6116.0007145
GAGCGAA2300.0113.5441741
TGCGTGA259.3877764E-487.00053145
AGCGAAA5700.080.178121
GCAAAAG46550.077.410723
TTAGGGC950.076.31625145
GCAGGGT9850.070.660339
CAAAAGC51850.069.348634
GAGCAAA53300.068.322961
AAGCAGG60700.064.020167
CGAAAGC7150.063.8853
GGCAGCC2100.058.69083145
ACAAGGG5350.058.2745633
TTTAAAC6100.058.241243
TAAACTA6100.058.2380565
CGGAGAT400.00605153954.375335
TACTGAT51850.052.4352267
CAAGGGT6100.052.2911034
AGCAGGC2650.051.981448
Produced by FastQC (version 0.11.5)