FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_Exp1_Shed_Ind3_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_Exp1_Shed_Ind3_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1195217
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT27710.2318407452370574No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA25480.21318304542187735No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA24490.20490003070572121No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC23520.1967843496201945No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT19950.16691529655284354No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG19720.16499095980060524No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT18660.15612227737724613No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG18500.1547836083322108No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT18050.15101860164304892No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT17120.14323758781878104No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC16680.13955624794493385No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA16600.13888691342241619No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGATCCTTCATTACTCATGTCAAAG16100.13470357265668076No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG15620.13068756552157473No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA15510.12976723055311293No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT15300.12801022743150406No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT15060.12600222386395107No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC15010.1255838897873775No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA14520.12148421583695679No Hit
TCTCTGTACTGAGAACGTAGGTTGTATGCTGATTTGGCCCGCAGATGCCC14430.12073121449912444No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA14320.11981087953066263No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT14060.1176355423324802No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT13340.1116115316298212No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA13270.11102586392261825No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA13210.11052386303072997No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT13160.11010552895415644No Hit
CATATGGAGCATGCCCCAAGTATGTTAAGCAAAACACCCTGAAGTTGGCA12970.10851585946317698No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC12910.10801385857128873No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT12720.10642418908030926No Hit
CTCAATATGAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAGC12350.10332851691366506No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA12300.1029101828370915No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT12280.1027428492064621No Hit
CATATTGAGAGGGTCGGTTGCTCACAAGTCCTGCCTGCCTGCCTGTGTGT11970.10014917793170612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCAAA100.007082455145.058731
ACGTGCG100.007091321144.99805145
GAGCGAA2300.0116.6776661
AGCAGGC6600.090.074548
CTACTAA451.2331348E-680.554466
TTAGTAG1000.072.529361
CTATGTA2100.069.058243
AGCGAAA4250.068.262931
GCAGGGT9600.064.947049
TAAACTA2550.062.548175
TGTGGCG350.003566865362.142014145
GCAAAAG28950.060.112873
ACAAGGG4600.058.324193
AAGGGTG3750.057.9992145
CAAAAGC30000.057.9967884
TTTAAAC2650.057.461673
CAAGGGT4900.054.7418674
CGAAAGC5300.054.72543
TCGGTCG400.006051497554.374268145
TCTATGT2700.053.714212
Produced by FastQC (version 0.11.5)