FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_Exp1_Shed_Ind3_D2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_Exp1_Shed_Ind3_D2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1770691
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC36280.20489176259437697No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT33740.19054708020767036No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT30020.16953833277517083No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG28710.16214009107179062No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC28220.15937280982396138No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT27860.15733970523371948No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT27350.15445947373087682No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA27190.1535558716907693No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT26760.15112744120798038No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA26730.15095801582546023No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA26220.14807778432261753No Hit
CATATGGAGCATGCCCCAAGTATGTTAAGCAAAACACCCTGAAGTTGGCA26090.1473436076650302No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT25910.14632705536990925No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA25610.1446328015447077No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT25050.14147019440433142No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA24790.14000184108915673No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA24160.1364439080562334No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT24010.13559678114363263No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC23610.13333777604336386No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT23120.13057049479553462No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA21810.12317225309215442No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG21630.12215570079703347No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC21190.11967079518673783No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA21070.1189930936566572No Hit
GTCTAGTAGAATAAGCATCTATTGGACAATAGTTAAGCCGGGAGACGTAC20660.11667761342888172No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA19910.11244197886587778No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA19510.11018297376560902No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT18890.10668151586019244No Hit
CTATTGGGGGACCCTCATTGTGATGTTTTTCAAAATGAGACATGGGACCT18890.10668151586019244No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT18780.10606028945761851No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG18740.10583438894759166No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT18390.10385775948485647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2250.0116.045541
TTAGTAG608.276402E-1084.616551
AGCAGGC6150.074.270178
AGCGAAA5050.073.246571
GGCAGCC2350.064.78887145
ACAAGGG6400.063.4498563
CTACTAA808.089046E-963.4391026
AAGGGTG5400.063.103455
AGCTTAT2000.061.649191
TTATATA2150.060.694074
CAAGGGT6800.059.7023354
ATTAGTA502.0067723E-458.0227661
GCAGGGT11750.056.7673959
TATAGGC2050.056.5868037
CAAAAGC36300.056.518684
TAGGCAT2100.055.239499
GAGTTTG5150.054.90431145
CGAAAGC6900.053.5973973
CTTATAT2200.052.7375453
GGCGACC553.219278E-452.728607145
Produced by FastQC (version 0.11.5)