FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_Exp1_Shed_Ind3_D1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_Exp1_Shed_Ind3_D1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2385726
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT56020.23481321828240126No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA47370.19855591128235178No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC45920.1924780968141354No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG44550.18673561004071718No Hit
CATATGGAGCATGCCCCAAGTATGTTAAGCAAAACACCCTGAAGTTGGCA40370.16921473798751407No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC39740.16657403239097868No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT39660.16623870469618052No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA39630.16611295681063123No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA38220.16020280618981392No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT37570.15747826866957898No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT37380.1566818653944334No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT37080.15542438653894033No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT36900.15466989922564453No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA34360.14402324491580343No Hit
GTCTAGTAGAATAAGCATCTATTGGACAATAGTTAAGCCGGGAGACGTAC31750.1330831788730139No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA31250.1309873807805255No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT30140.12633470901520122No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT29850.12511914612155797No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT28560.11971198704293787No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC27970.11723894529380155No Hit
CTCCATATGTGATCTTGTTTACGTTTTGAAAGGGCTTGTCATTGGGAATG26940.11292160122327542No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA26770.11220902987182936No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT26500.11107729890188563No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA26490.11103538294003586No Hit
CTGCTGTTCCTTTCGATATTCTTCCCTCATAGACTTTGGCACTCCTTCCG25830.10826892945795116No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA25570.10717911444985719No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA25440.10663420694581022No Hit
CTATTGGGGGACCCTCATTGTGATGTTTTTCAAAATGAGACATGGGACCT25210.10567013982326554No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA24520.10277793845563153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA3700.099.978451
TCGGTCG750.086.99993145
GGACTAC300.00193575772.5029756
AGCGAAA6600.068.137421
ACAAGGG10300.065.473463
AAGGGTG8950.063.186955
CAAGGGT11250.059.930774
GCAGGGT20750.059.0506139
GGCAGCC4450.058.651638145
ACGTGCG502.0108199E-457.99995145
GCTCACG502.0108199E-457.99995145
TAAACTA3400.057.5758935
TTTAAAC3450.056.7497863
GGCGACC1051.1095835E-955.23805145
CTTCCCG953.1419404E-853.421005145
TATAGGC3550.053.100777
TTATATA3550.053.0963174
CTACTAA1101.6771082E-952.729446
CAAAAGC41700.051.2867744
AGGTCGT1700.051.1785746
Produced by FastQC (version 0.11.5)