FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_INFANT_Exp1_Shed_Cnt5_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_INFANT_Exp1_Shed_Cnt5_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1296372
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC143111.1039269592370091No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA77520.5979765067434348No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA64420.49692526527879344No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT60300.46514426414640236No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT40950.31588155251733296No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA36530.2817864008170494No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA36390.28070646388536624No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG33140.2556364993998636No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA31670.24429716161719012No Hit
CCATTTGAATGGATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCA27880.2150617261094809No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC27030.2085049661671187No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTTGGAGCCAGGATAC26540.2047251869062275No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT26110.20140823775891487No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA24940.19238305054413396No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT24630.1899917616239783No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG24400.18821757952192736No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC22400.17278990906931035No Hit
AAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTTGCCG22340.17232707895573185No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG22330.17224994060346877No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT21730.16762163946768366No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA21590.16654170253600048No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA21330.16453610537716026No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA21170.1633018917409509No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG21000.16199053975247846No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT20960.16168198634342612No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG20790.1603706343549537No Hit
CACTTGGAAGTATGCTTCATGTATTCAGGATTTTCAGCTGAATCAAGAAA20610.15898214401421812No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA20530.15836503719611347No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA19550.15080547867433114No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT19490.1503426485607526No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT19280.14872274316322784No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT19190.14802849799286008No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT18780.14486582555007357No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG18420.14208884486860254No Hit
GGACAAGCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAA18290.14108604628918242No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT18040.13915758748260532No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA17550.13537780822171414No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA17330.13368076447192628No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT17310.13352648776740011No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC16730.12905246333614118No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA16360.12619834430240703No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGA16310.12581265254109159No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT16270.12550409913203925No Hit
GATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGATTGTC16130.12442416220035607No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG15690.12103007470078034No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA15570.1201044144736233No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA15380.1186387857806247No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT15330.11825309401930927No Hit
TCTTAAAAGTGCCAGCACAAAATGCTATAAGCACAACTTTCCCTTATACT15290.11794454061025694No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA15140.11678746532631065No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC15120.11663318862178448No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC15060.11617035850820599No Hit
GTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTTT14920.11509042157652279No Hit
GATCTTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGA14830.11439617640615503No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA14630.11285340936089332No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT14560.11231344089505174No Hit
ATGTCAATCCGACCTTACTTTTCTTAAAAGTGCCAGCACAAAATGCTATA14520.11200488748599939No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT14470.11161919572468396No Hit
ATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGATTGTCGAGCTTGC14380.11092495055431618No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA14360.11077067384979004No Hit
TAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGA14110.10884221504321291No Hit
TTCATGATCTCAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATC14010.10807083152058206No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC13990.10791655481605589No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA13970.1077622781115297No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG13860.10691375623663579No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC13830.10668234117984653No Hit
CTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAAC13820.10660520282758344No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT13770.10621951106626801No Hit
GTGGAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCT13710.1057566809526895No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT13680.10552526589590026No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA13600.10490815907779558No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA13590.10483102072553249No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG13570.10467674402100631No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG13480.10398249885063854No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT13390.1032882536802708No Hit
CATCAGTACTCAGAAAAGGAAGATGAACAAATGTACCAAAGGTGCTGCAA13350.10297970027121844No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATATT13320.10274828521442919No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA13320.10274828521442919No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC13130.10128265652143058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4750.0109.9395451
GCAGGTA20900.098.896312
GGTACTG21700.095.228315
CAGGTAC21900.094.365943
AGCAGGT23250.088.90721
GAGCGAA1300.083.687961
TCCCACG451.2335004E-680.5511145
CGAAAGC6550.079.7086943
ATTATAT8450.074.67541
GTAGAAA20500.073.2315142
CAGGGCG300.001935408372.504389
AGGTCCG300.00193629772.49599145
GTCTAGG808.06358E-963.463361
TTGGCCG350.003567523662.13942145
CAAGGCA16350.062.0832639
AGAAACA24750.060.9259224
GTACAGC502.0071861E-458.019172
ATAGATC502.008718E-458.0102163
GGACAGT21850.057.406216
ACTGATC38850.056.921078
Produced by FastQC (version 0.11.5)