FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_HBEpC_LOW_3DPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_HBEpC_LOW_3DPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112957
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT34800.3126805438125642No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA23800.2138447397338801No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG23040.20701608417935283No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT22740.20432056224993417No Hit
GTTTTCATAACCTCTTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA22710.20405101005699233No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC21290.191292206257744No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT19780.1777247458796701No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG18240.16388773330865433No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17220.1547229587486309No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA16470.14798415392508424No Hit
GGTTTCAGCTTTTCAAGTTCTTCAATGGTTTTGAAATCTCCAAGATCTGG16360.14699579588429743No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT15000.13477609647093283No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT14980.1345963950089716No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA13420.12057968097599459No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATATATCACCCAGG13180.11842326343245964No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT13100.11770445758461467No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT13090.11761460685363405No Hit
GTCCTAACTATATGCGGCAGAATGATGTCATTAAAAGGGAAAATTGAAGA11770.10575431036419197No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAAGAGG11760.10566445963321135No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT11510.10341819135869579No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG11340.10189072893202523No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT11320.10171102747006397No Hit
GTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATCCTCTT11280.1013516245461415No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGT100.0070852884145.038861
CGGGACG100.007089097145.01286
CGGTTCC100.007091002144.999774
CCGTGTG100.007091955144.99326145
GTAACAA13850.0127.725444
CACGCAC2950.0120.4343643
GCACGCA3100.0114.6223452
TAGAAAC5450.0114.429212
AAACACG5450.0111.752995
AGCACGC3200.0111.045391
GAAACAC5500.0110.72714
TAGTAAC16400.0108.774272
CGCACTT3850.092.280875
ACGCACT4100.088.414494
ACGTGCA2650.082.093792
ACAAGAG21950.079.938737
AGTAACA22600.079.243733
AACACGT1100.079.09796
AACACGA6750.077.3401646
CGGTGCG950.076.3328252
Produced by FastQC (version 0.11.5)