FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_HBEpC_LOW_3DPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_HBEpC_LOW_3DPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376083
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA36950.2685157799347859No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT34460.2504209411786934No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT33600.24417131815450085No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA25910.18828806111259278No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG24680.1793496467872941No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG24240.17615216524003277No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT23210.16866715161803467No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC21970.15965606725757095No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT20840.15144435328392256No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA20110.14613944071687535No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATATATCACCCAGG19670.14294195916961402No Hit
ATACAGAAGATTGGAATATGGGACGGGGAAGAGGAGTTCCATGTAAGATG18450.13407621487948038No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17910.13015203298056877No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17830.12957067269924852No Hit
GTATAGTCAGATGCATTCATTGATTCATTTATCCCATGTAGTTCACTTGC17320.12586450090583198No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG17150.12462911030802648No Hit
GTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATCCTCTT17130.12448377023769643No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG15950.11590870608822287No Hit
CTTCTGTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATC15860.1152546757717376No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT15590.1132925848222818No Hit
GTTTTCATAACCTCTTGGTCTCTCATTTGTTTGAGCACTGCTCTTGCAAA15470.11242054440030143No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA15430.1121298642596413No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT15290.1111124837673309No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG15150.11009510327502046No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT15070.10951374299370024No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA15020.10915039281787509No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT14410.10471752067280826No Hit
GAACAATACAGAAGATTGGAATATGGGACGGGGAAGAGGAGTTCCATGTA14360.1043541704969831No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA14150.10282809975851748No Hit
CATTTATCCCATGTAGTTCACTTGCTTTGGGTGATTCCTCATACCCCCAT14070.10224673947719723No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAAGAGG14050.10210139940686716No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAA13870.10079333877389662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA14150.0130.645724
CTGTCCG256.4677097E-6115.992905145
TAGAAAC6250.0112.533562
GAAACAC6150.0111.9850164
GCACGCA2750.0110.740622
AAACACG6350.0109.6155245
AGCACGC2800.0108.778921
CACGCAC2850.0106.843343
TAGTAAC17600.0104.6432952
CGCACTT3250.093.70025
ACGCACT3450.088.255484
GCCGACC353.43878E-582.85208145
ACAAGAG23750.078.459247
AACACGA7200.075.527196
AACACGT2050.074.274546
ACACGAG7400.073.485927
AGTAACA25750.072.923173
TAACAAG28850.064.086855
ACGTGCA3050.061.810732
CGGAGCA1007.185008E-1058.0069922
Produced by FastQC (version 0.11.5)