FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_HBEpC_LOW_2DPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_HBEpC_LOW_2DPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934272
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT17920.1918070968625839No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA17380.18602719550623373No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT17300.18517091382381148No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT15820.16932970269899986No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG15330.16408497739416358No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT13400.1434271818057268No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT12930.1383965269214961No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG12480.13357994245787094No Hit
ATACAGAAGATTGGAATATGGGACGGGGAAGAGGAGTTCCATGTAAGATG12200.13058295656939306No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT12190.1304759213590903No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA12160.13015481572818194No Hit
GTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATCCTCTT12130.1298337100972736No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA11970.1281211467324291No Hit
CTTCTGTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATC11650.1246960200027401No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC11330.1212708932730511No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA11040.11816687217427045No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT10860.11624023838882039No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT10330.11056737224277298No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATATATCACCCAGG10310.11035330182216742No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT10130.10842666803671736No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT9920.10617892862035895No Hit
GTATAGTCAGATGCATTCATTGATTCATTTATCCCATGTAGTTCACTTGC9910.10607189341005617No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT9570.1024326962597616No Hit
ATCTATCACAGTCTTGTTTGCAATTTCTACAGCTAGCTCCAGTGGGTTTG9350.10007792163310042No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGT100.0070851543145.038565
CACGCAC2500.0136.321643
GCACGCA2550.0133.655822
AGCACGC2600.0131.099551
GTAACAA9350.0126.376614
CGCACTT2900.0120.03195
GAAACAC4050.0107.395724
ACGCACT3250.0107.0652544
TAGTAAC11700.0102.8850862
TAGAAAC4300.0102.870682
AAACACG4250.0102.380155
AACACGT1100.079.111936
AGTAACA16250.074.519523
TAACAAG15950.073.6559455
AACACGA4800.072.519286
CTCCGTA300.001936127372.49599145
ACGTGCA1900.068.698792
ACACGAG5200.066.937297
ACAAGAG18550.062.937897
CAGCATC702.3361281E-762.1360864
Produced by FastQC (version 0.11.5)