FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_HBEpC_LOW_2DPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_HBEpC_LOW_2DPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362355
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT17620.1293348649948068No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA16590.12177442737025225No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG16190.11883833508887183No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT16120.11832451893963028No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT16100.11817771432556126No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA15210.11164490899948985No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT15090.11076408131507573No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT14400.10569932212969455No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA13870.1018089998568655No Hit
ATACAGAAGATTGGAATATGGGACGGGGAAGAGGAGTTCCATGTAAGATG13810.10136858601465845No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT13660.10026755140914079No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCCCG100.0070900517145.007467
GTAACAA7850.0116.3625954
GAAACAC5000.0107.29374
AAACACG5250.0102.1957245
GCACGCA1850.0101.934542
CACGCAC1850.0101.919573
AACACGT1000.094.254846
TAGAAAC6600.089.014512
CGTGCCT900.088.6351853
AGCACGC2200.085.7428741
CACGTGC3300.079.147261
ACACGTG1200.078.545717
ACGTGCA2350.077.159952
AACACGA6000.072.503726
ACACGAG6300.069.051177
TAGTAAC13800.066.748952
GAAGCGG1350.064.4903641
ACGTGCC1250.063.8267022
CAACGCA2600.061.3493089
CGCACTT3800.059.1477785
Produced by FastQC (version 0.11.5)