FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_HBEpC_LOW_1DPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_HBEpC_LOW_1DPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894032
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT32770.17301714015391503No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT29600.15628035851559002No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT25670.1355309730775404No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT25460.13442222729077438No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT25450.13436942987235695No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA23680.12502428681247202No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT21690.11451760054740363No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC20150.10638679811111956No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT20020.10570043167169299No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG20010.10564763425327556No Hit
GTGTAAACACAATAAGTAATGAGGAAACGAGTAACGAGCTACAGAAAACC19240.1015822330351335No Hit
CATATGGACCAATAGGAGATACTGAAGGTTTCGAAAGGGTCTACGAAAGC19150.10110705626937666No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCACG151.2303947E-4144.99358145
GTAACAA17600.0122.338344
GCACGCA5650.0119.362522
CACGCAC5850.0115.269573
AGCACGC5950.0113.36221
GAAACAC8300.0111.802294
ACGGCGG203.8672777E-4108.745186145
CGCACTT6600.0105.461015
AAACACG8900.0104.276075
TAGAAAC9350.0102.375442
ACGCACT7150.096.32444
TAGTAAC22750.095.306662
ACGTGCA3800.083.965822
AACACGT2250.083.782916
GAGCATC2450.076.94356
GAAGCGG2250.074.1391451
CACGTGC5350.073.205251
TGTGCCG300.001936350572.49679145
ACACGTG2650.071.136447
AACACGA10600.070.452446
Produced by FastQC (version 0.11.5)