FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_HBEpC_LOW_1DPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_HBEpC_LOW_1DPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1122188
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA18890.1683318659618531No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT17570.15656913101904493No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT15760.14043992628686103No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT14790.1317960983364641No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG14040.12511272620986857No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT13980.12457805643974094No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG13500.1203006982787198No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT12950.1153995587192164No Hit
ATACAGAAGATTGGAATATGGGACGGGGAAGAGGAGTTCCATGTAAGATG12880.11477577732073414No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA12760.11370643778047884No Hit
GTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATCCTCTT12220.10889440984933006No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA11990.1068448423971741No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT11920.10622106099869184No Hit
CTTCTGTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATC11870.10577550285691881No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACTGGCAAGA11770.10488438657337273No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT11700.10426060517489048No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT11300.10069614004070619No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCCG100.0070897583145.008335
GCACGCA2850.0122.128622
GTAACAA7850.0120.983444
CACGCAC2950.0117.967623
AGCACGC3100.0112.304561
GAAACAC4200.098.389754
AAACACG4300.096.1101765
CGCACTT3850.092.278035
TAGAAAC4750.091.596462
ACGTGCA2250.090.239472
ACGCACT4250.085.291424
GAAGCGG950.083.982131
TAGTAAC11550.082.8729862
CACGTGC3200.079.329711
GGGGTGC502.3068587E-672.4977145
CTGTCCG451.1941087E-464.442406145
AACACGA5300.062.9281436
CGATCTG350.003565804662.146435
CCGATCT350.003567063762.1408924
ACACGAG5500.060.6398477
Produced by FastQC (version 0.11.5)