FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_HBEpC_LOW_12HPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_HBEpC_LOW_12HPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1878299
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC163840.8722785882332897No Hit
GTAGTAACAAGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA107150.5704629561108215No Hit
GAGTATGGATGGTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTA61460.3272109499073364No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT61020.32486840487057705No Hit
GTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATA60750.3234309340525657No Hit
AAATAAAGGTATGTGTGCTTCACAACTAGTTATATTTACAAATAGTGTCA58410.31097285362980015No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT49870.26550618405269877No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA49700.2646011098339508No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA44270.23569197449394372No Hit
GGTCTTAATTGAATCAATCCATTAAAGCATTAGCAGATGCTTTATGGTAT44070.23462718129541676No Hit
GTAGAAACACGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA40680.21657893658038468No Hit
GTAATGGTAAGAAATCTTTCGGGTAAGAGTATGGATGGTTCTTCTTTTAC40490.21556738304178408No Hit
TTGTAGAACCATGCTGCAAAGCCATATACCGATGGGGTTATCCATGCAAC39150.20843326861165343No Hit
ATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATTTGCCC38950.20736847541312645No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT38160.20316254227894492No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA37180.1979450556061628No Hit
GCTTATAGCTTTTTTTGTAAACAGGGAAATGTTGAGTGGTACTTGTATTC35590.18947994967787343No Hit
CCTACAATGTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCC33990.18096160408965772No Hit
GTACTTGTATTCCCTTATCAAATTAATCACATAGCTTTTATACAGAAACT33490.17829962109334033No Hit
CCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATT33390.17776722449407684No Hit
GTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCATTAGCAGATGC33000.17569087775694925No Hit
TTCCTACAATGTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACT32340.17217706020181026No Hit
CTCTAACAGTAAACTTTTGGGAATTTTTGAGCTACATATATGAATCCCTG32090.17084606870365154No Hit
TCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCCCAAAATAAAGG30460.1621680041356568No Hit
GAAATAGTGATAGGAAATTGCCAACTCTAACAGTAAACTTTTGGGAATTT30020.15982545909889748No Hit
GTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTAAACAGGGAAAT29610.15764263304191717No Hit
GGGTTATCCATGCAACTATCTCCATTCCTACAATGTCCTTAATATGAGAT29100.15492741038567343No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA27530.14656878377723673No Hit
CCATAAAGCATCTGCTAATGCTTTAATGGATTGATTCAATTAAGACCTGA27360.14566370955848884No Hit
TACAAATAGTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCATTA27150.1445456767000355No Hit
CCATGGAATATTTGCCCAGTAATGGTAAGAAATCTTTCGGGTAAGAGTAT26540.14129805744452828No Hit
CATTGATAGTAGGGACTCCATGGAATATTTGCCCAGTAATGGTAAGAAAT26260.13980734696659053No Hit
ATAGTAGGGACTCCATGGAATATTTGCCCAGTAATGGTAAGAAATCTTTC25410.13528197587285093No Hit
GCTTTTATACAGAAACTCTTCCTGTGTGAGCTGTTAAGGTGCCAAATTGG24830.1321940755971227No Hit
CTTCACAACTAGTTATATTTACAAATAGTGTCACTCAGGTCTTAATTGAA22630.12048135041332611No Hit
ACTATCTCCATTCCTACAATGTCCTTAATATGAGATCATCAAACATTGAT21770.11590273965966015No Hit
TTTACAAATAGTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCAT21500.11446526884164876No Hit
AACACGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGATAGGA21140.11254864108430021No Hit
CTTTTACCCAATCTGCTTATAGCTTTTTTTGTAAACAGGGAAATGTTGAG20600.1096736994482774No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG19770.1052548076743905No Hit
GGGAAATGTTGAGTGGTACTTGTATTCCCTTATCAAATTAATCACATAGC19510.10387057651630545No Hit
CTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATTT19470.10365761787660005No Hit
TACAAGTACCACTCAACATTTCCCTGTTTACAAAAAAAGCTATAAGCAGA19460.10360437821667369No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA19210.10227338671851499No Hit
CTGAAATAGTGATAGGAAATTGCCAACTCTAACAGTAAACTTTTGGGAAT19190.1021669073986623No Hit
TGTATTCCCTTATCAAATTAATCACATAGCTTTTATACAGAAACTCTTCC18930.10078267624057725No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC13300.0119.988331
TAGTAAC22900.0115.9100342
CACGCAC13650.0115.808773
GCACGCA13850.0114.1516342
CGCACTT15050.0108.4027945
ACGCACT15350.0105.32544
AAACACG13800.094.577515
GAAACAC15600.084.118334
AACACGT4700.083.306486
TAGAAAC17450.079.380412
ACACGTG4950.079.096987
GCACTTC15900.077.979766
AGTAACA34950.076.766573
GTAACAA35050.075.499054
GTAGTAA37050.072.8320851
AAGAGCC25850.068.1580359
ACAAGAG41900.063.507267
AACACGA14700.062.642536
AGCGCGA350.003565906362.147638
CACGAGC15350.059.988318
Produced by FastQC (version 0.11.5)