FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_HBEpC_LOW_12HP1_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_HBEpC_LOW_12HP1_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1398706
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACATATATGAATCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCC127380.9106988888301044No Hit
GTAGTAACAAGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA86190.6162124134735963No Hit
GCCATATACCGATGGGGTTATCCATGCAACTATCTCCATTCCTACAATGT54570.39014632095665563No Hit
GAGTATGGATGGTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTA48310.34539066823192294No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA48270.3451046896202633No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT44730.3197955824883857No Hit
GTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATA38900.27811419983899405No Hit
AAATAAAGGTATGTGTGCTTCACAACTAGTTATATTTACAAATAGTGTCA37340.26696103398426835No Hit
AGCACGCACTTCCTCCTGGGCTAGGGGTTGTCAGTGAGGTTTCAACATTT35290.25230463013671206No Hit
TTGTAGAACCATGCTGCAAAGCCATATACCGATGGGGTTATCCATGCAAC32150.22985530912143082No Hit
GGTCTTAATTGAATCAATCCATTAAAGCATTAGCAGATGCTTTATGGTAT29970.21426947478598077No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA28880.20647655761825576No Hit
GTAGAAACACGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGA28360.2027588356666805No Hit
GCTTTATGGTATGTGTCATGAATACAAATGACAGCCAATTTGGCACCTTA27270.19496591849895545No Hit
CTCTAACAGTAAACTTTTGGGAATTTTTGAGCTACATATATGAATCCCTG27260.19489442384604055No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG27160.19417947731689147No Hit
ATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATTTGCCC26120.186744033413741No Hit
GTAATGGTAAGAAATCTTTCGGGTAAGAGTATGGATGGTTCTTCTTTTAC25610.18309780611508064No Hit
GGGTTATCCATGCAACTATCTCCATTCCTACAATGTCCTTAATATGAGAT25020.17887962159310106No Hit
TCCCTGATCTGTGGTTTTCTATATTTTCTCATAAATTCCCAAAATAAAGG24520.17530488894735563No Hit
GTATAAAAGCTATGTGATTAATTTGATAAGGGAATACAAGTACCACTCAA23700.16944232740833315No Hit
TTCCTACAATGTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACT22810.16307930329890627No Hit
GAAATAGTGATAGGAAATTGCCAACTCTAACAGTAAACTTTTGGGAATTT22320.15957606530607574No Hit
GCTTATAGCTTTTTTTGTAAACAGGGAAATGTTGAGTGGTACTTGTATTC22020.1574312257186285No Hit
GTACTTGTATTCCCTTATCAAATTAATCACATAGCTTTTATACAGAAACT21480.15357051446122344No Hit
CCTACAATGTCCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCC21070.1506392336917122No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT21040.15042474973296746No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG20910.14949531924507367No Hit
GTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCATTAGCAGATGC20630.14749346896345622No Hit
CCTTAATATGAGATCATCAAACATTGATAGTAGGGACTCCATGGAATATT20350.1454916186818388No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG20290.14506265076434935No Hit
GTTCTTCTTTTACCCAATCTGCTTATAGCTTTTTTTGTAAACAGGGAAAT19750.14120193950694426No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA18900.13512489400917704No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA18560.13269407581007014No Hit
CCATGGAATATTTGCCCAGTAATGGTAAGAAATCTTTCGGGTAAGAGTAT17710.12661703031230293No Hit
ATAGTAGGGACTCCATGGAATATTTGCCCAGTAATGGTAAGAAATCTTTC16970.12132642599659972No Hit
CATTGATAGTAGGGACTCCATGGAATATTTGCCCAGTAATGGTAAGAAAT16760.11982503828538663No Hit
CTGAAATAGTGATAGGAAATTGCCAACTCTAACAGTAAACTTTTGGGAAT16230.11603582168089649No Hit
TACAAATAGTGTCACTCAGGTCTTAATTGAATCAATCCATTAAAGCATTA15770.1127470676468107No Hit
AACACGAGCCATTATTTTCAGTTTGTTCTGACACTGAAATAGTGATAGGA15740.11253258368806597No Hit
ACTATCTCCATTCCTACAATGTCCTTAATATGAGATCATCAAACATTGAT15510.11088820667102307No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG15180.10852888312483108No Hit
CTTCACAACTAGTTATATTTACAAATAGTGTCACTCAGGTCTTAATTGAA14610.10445368790868131No Hit
CCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAAAAGCTAAA14410.10302379485038315No Hit
CTTTTACCCAATCTGCTTATAGCTTTTTTTGTAAACAGGGAAATGTTGAG14330.10245183762706386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC8100.0124.493241
TAGTAAC18000.0122.4877852
GCACGCA8300.0122.332442
CACGCAC8550.0120.439053
CGCACTT9000.0113.59515
ACGCACT9000.0113.591044
GAAACAC9650.0101.4317554
GTAACAA21700.0101.239584
AAACACG10050.097.398155
GCACTTC10500.092.526786
AACACGT3400.085.296856
TAGAAAC11600.085.030082
AGTAACA28200.078.946763
ACAAGAG28050.077.284117
AAGAGCC20000.076.489959
ACACGTG3800.076.318247
GTAGTAA30700.073.255341
AAGCGCC406.663056E-572.502339
AACACGA10550.066.660916
CGGTGCG451.1919347E-464.4672552
Produced by FastQC (version 0.11.5)