FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_HBEpC_HIGH_1DPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_HBEpC_HIGH_1DPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014128
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT86960.4317501171722949No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT69020.34267931333063245No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT49680.24665761063845No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT42640.2117045192758355No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT35860.17804230912831756No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT34750.17253123932540534No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC32420.16096295766704002No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT32400.1608636591120326No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG32130.15952312861943233No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT28680.1423941278806511No Hit
ATATAAATCCTTATTTTCTCTTCATAGATGTGCCCGTACAGGCAGCAATT28630.1421458814931325No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT28140.13971306689545054No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG27590.13698235663274627No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC26680.13246427237990832No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA26040.12928671861967064No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA25810.12814478523708522No Hit
CTTCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACA24980.12402389520427698No Hit
GTAGAAACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT24620.12223652121414329No Hit
AACACGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTATTCAAT24560.12193862554912101No Hit
ATTCAATGATGACCAATAACCCCATAAACATCTTCGAAGCTTATATGTAC22410.11126403088582255No Hit
GTGTGAATGTGATGCTTGTTTCTCGCATAAAGCACAGAGCGTTCCTAGTT21720.1078382307380663No Hit
TCATAATCCTCTGCTGTGTCCCTCCCAAAGAAGAAATTGGGGATGGTCTG21660.107540335073044No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA29600.0127.6078954
CACGCAC11100.0119.5430453
AGCACGC11750.0113.575331
GCACGCA11800.0113.077242
TAACAAG34750.0109.129745
GAAACAC11850.0108.289534
AAACACG12700.0102.198745
TAGAAAC13600.098.644352
CGCACTT14050.098.571945
ACGCACT14500.092.498624
TAGTAAC41550.090.929762
ACAGCGT406.120281E-790.623664
AGTAACA46200.082.083513
AACACGT3800.076.3222356
ACGTGCA5150.074.629072
GAAGCGG3250.071.411921
AACACGA15000.067.672386
CCGCACT651.398912E-766.922084
AGACGGC651.3996396E-766.9171145
GCACTTT22450.066.208276
Produced by FastQC (version 0.11.5)