FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_DVG_TEST_COND2_0.1_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_DVG_TEST_COND2_0.1_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2519796
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG147070.5836583596449871No Hit
GGATGTAATCATGGAAGTTGTTTTCCCTAACGAAGTGGGAGCCAGGATAC129830.5152401226131005No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA123660.490754013420134No Hit
GTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGT122070.48444397879828366No Hit
AACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAG111090.44086902273041156No Hit
TCCTTAGGCAGAACCCAACAGAAGAGCAAGCCGTGGATATATGCAAGGCT100130.39737343816721676No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACCAATCTGTGTGCTGTGGCAC99270.39396046346609015No Hit
ATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAA93310.370307755072236No Hit
ATTACATCCTGTGCCTCCTTGGCACTGAGATCTGCATGACCAGGATTTAT88930.35292539554789354No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA86410.34292458595854586No Hit
GATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCT83940.3331222051308915No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA82440.3271693422800893No Hit
GTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAAT76920.3052628069891372No Hit
GTATTTGTTATTGGTCCATTCCTATTCCACCATGTCACAGCCAGAGGTGA76440.3033578908768805No Hit
GTATTGGCCTCTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATT70100.2781971238941565No Hit
GAATGAGGAATCCCCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCA64200.2547825300143345No Hit
GGATACTAACATCGGAATCGCAACTAACGATAACCAAAGAGAAGAAAGAA63670.25267918514038434No Hit
CTTCATATCCCTCATGCACTCTTATCTTCAATGTTTGAAGATTGCCCGTA62170.24672632228958216No Hit
GCATATATCCACGGCTTGCTCTTCTGTTGGGTTCTGCCTAAGGATGTCTA61670.2447420346726481No Hit
CTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTT60810.24132905997152151No Hit
GTTCATTGATGCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGA60430.23982100138265158No Hit
TAATAAAACTTCTTCCCTTCGCAGCCGCTCCACCAAAGCAAAGTAGAATG58950.23394751003652675No Hit
TAACAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGA58610.2325981944570116No Hit
GCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTG56790.2253753875313716No Hit
CTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGATTATT56770.22529601602669422No Hit
GTGTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAG54750.2172794940542806No Hit
CTCCTGGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAAC53520.21239814651662275No Hit
GTGGAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCT52810.2095804581005764No Hit
GGCTAAAGCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAA52600.20874705730146412No Hit
GTGTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGAT51650.20497691082928937No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT50140.1989843622261485No Hit
CTACAATGCTATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTA49920.19811127567469747No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC48900.19406332893615197No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG48550.19267432760429812No Hit
ATGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTTTTGCG48150.19108689751075086No Hit
GTATGCAACCATCAAAGGAGAAATTTTGCAATCCTGGAGTTCTTCTTTCT47740.18945978166486493No Hit
CTAATGGCCTTAGGTACTAAAGACTGAAATGGTTCAAATTCCATTTTATT47190.18727706528623744No Hit
GTATCAGCAGATCCACTAGCATCTTTATTGGAGATGTGCCACAGCACACA46850.1859277497067223No Hit
CCTCAGAACAGCGGACTCCACTCCAGCTGTGCCTTCATCTGGGTCTTCAG46230.18346723306172405No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC45960.18239571774857966No Hit
CCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTT45940.18231634624390228No Hit
GTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATCCCCTC45800.18176074571116074No Hit
GGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGGAATAG45690.18132420243543526No Hit
GTTAGTATCCTGGCTCCCACTTCGTTAGGGAAAACAACTTCCATGATTAC45650.18116545942608053No Hit
GTATCAAATGTCCCAAGCACATCCCTCATTTGTTGGAACAGAGTTCTTAC45600.1809670306643871No Hit
CTTTAGTACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACT45580.18088765915970975No Hit
GTTTTATTATCTGTGCGGTATCAAATGTCCCAAGCACATCCCTCATTTGT45120.1790621145521304No Hit
TCTGTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATCTGCTGAT44750.17759374171559922No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG44530.17672065516414823No Hit
GCTTAGTGCTGGCCCATATCTCTTGTCTTCTTTGCCCAGAATGAGGAATC44120.17509353931826227No Hit
GGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTC44030.17473636754721414No Hit
GTAGTACATTTCCTCGTTGGTCCCGGATTCTCAAAAAACGGTCAATGCTC43970.17449825303318206No Hit
CTCTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTC43960.17445856728084336No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC43610.1730695659489895No Hit
GATTCAGTCCCATTGCAGCCTTGCATATATCCACGGCTTGCTCTTCTGTT43380.17215679364519984No Hit
CCACTACCCTCTCCGTGCTGGAGTACTCATCTACACCCATTTTGCTGATT43230.17156150736011963No Hit
GATATATGCAAGGCTGCAATGGGACTGAGAATTAGCTCATCCTTCAGTTT42850.17005344877124975No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA42820.1699343915142337No Hit
GGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAATGAGGGATGT42120.16715638885052597No Hit
GATATGAAGAGTTCACAATGGTTGGGAGAAGAGCAACAGCCATACTCAGA41550.16489430096722116No Hit
TTATTGTATAGCATTGTAGGGTTCTGGGACCACTGAATTTTAACAGTTTC41510.16473555795786643No Hit
CATAAATCCTGGTCATGCAGATCTCAGTGCCAAGGAGGCACAGGATGTAA41410.1643387004344796No Hit
ATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGAT40330.16005263918190202No Hit
ATTTGATACCGCACAGATAATAAAACTTCTTCCCTTCGCAGCCGCTCCAC40270.15981452466786994No Hit
GTCCATTTTAGAAACCAAGTCAAAATACGTCGGAGAGTTGACATAAATCC40220.15961609590617654No Hit
TCAATGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTTTT40210.15957641015383786No Hit
ATGTTGGAGAGAGAACTGGTCCGCAAAACGAGATTCCTCCCAGTGGCTGG39740.15771117979391983No Hit
TTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTCTACTT39650.1573540080228717No Hit
CAAATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAGTC39560.15699683625182356No Hit
GTTCCTAGCAGCAATAATCAAGCTTTGATCAACATCATCATTCCTCACTT39320.1560443781956952No Hit
CAATTATTGCTTCGGCAATCGACTGTTCGTCTCTCCCACTCACTATCAGC39320.1560443781956952No Hit
GGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCCGGGACCAACGAGGA38830.15409977633109984No Hit
TCCTAATTCCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCT38780.1539013475694064No Hit
ATACAATAAAATGGAATTTGAACCATTTCAGTCTTTAGTACCTAAGGCCA38550.15298857526561674No Hit
GTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAA38300.1519964314571497No Hit
CTTATCTTCAATGTTTGAAGATTGCCCGTAAGCACCTCTTCCTCTCTCTT38050.15100428764868268No Hit
GTTCTGGGACCACTGAATTTTAACAGTTTCCCAGTTTCTGATGATCCATT37990.1507661731346506No Hit
GTACTAAAGACTGAAATGGTTCAAATTCCATTTTATTGTATAGCATTGTA37680.14953591481215145No Hit
ATGCAGGATCAGACCGAGTGATGGTATCACCTCTGGCTGTGACATGGTGG37640.14937717180279672No Hit
GATAAAAGCAGTCAGAGGTGATCTGAATTTCGTCAATAGGGCGAATCAGC37200.14763099869989474No Hit
GTATTGACCAACACTGATTCAGGACCATTAATCTCCCACATCATTGACGA37110.14727382692884664No Hit
CTTCAATGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTT37100.14723414117650793No Hit
ATACCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGG36720.14572608258763806No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT36480.14477362453150971No Hit
ATCCCAATCATTCCCATCACATTGTCGATAGGTTCAACTCCCCAATTTTG36460.14469425302683234No Hit
GGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCTACAAAACT36030.14298776567626903No Hit
AATGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTTTTGC36010.14290839417159168No Hit
ATGTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAA35240.1398525912415132No Hit
GAATAGGAATGGACCAATAACAAATACAGTTCATTATCCAAAAATCTACA35160.13953510522280374No Hit
GTCGATAGGTTCAACTCCCCAATTTTGAAAAAGCACTTTCGCATCCTTCT34920.1385826471666754No Hit
GGATTTATGTCAACTCTCCGACGTATTTTGACTTGGTTTCTAAAATGGAC34770.1379873608815952No Hit
ATTGAATCCTATGCATCAACTTTTAAGACATTTTCAGAAGGATGCGAAAG34730.13782861787224046No Hit
AGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAAGA34590.1372730173394989No Hit
GTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCC34460.13675710255909604No Hit
GCTGATACTGCAGCTCTTCTCACTATGTTCCTAGCAGCAATAATCAAGCT34410.13655867379740266No Hit
TTTCAGAAGGATGCGAAAGTGCTTTTTCAAAATTGGGGAGTTGAACCTAT33900.1345347004281299No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG33880.13445532892345252No Hit
CATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAATGTACA33830.13425690016175912No Hit
GTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCAAAGCTTGA33590.13330444210563078No Hit
TCTCAGTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTA33310.1321932410401477No Hit
CTCTTGACTGATGATCCGCTTGTTCTCTTAAATGTGAATCCACCAAAACT32910.13060581094660043No Hit
CATCAGGAAGACAGGAGAAGAACCCAGCACTTAGGATGAAATGGATGATG32860.13040738218490702No Hit
GAAGATAAGAGTGCATGAGGGATATGAAGAGTTCACAATGGTTGGGAGAA32600.12937555262410133No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT32580.12929618111942395No Hit
GTTCTCTCTCCAACATGTATGCAACCATCAAAGGAGAAATTTTGCAATCC32490.12893900934837582No Hit
ACTTTATGGAGTAAAATGAATGATGCAGGATCAGACCGAGTGATGGTATC32320.12826435155861823No Hit
GAATCAGTGTTGGTCAATACCTATCAATGGATCATCAGAAACTGGGAAAC32280.1281056085492635No Hit
GTAGAATGCAGTTCTCCTCATTTACTGTGAATGTGAGGGGATCAGGAATG32000.12699440748378044No Hit
GCATGGAACCTTTGGCCCTGTCCATTTTAGAAACCAAGTCAAAATACGTC31980.12691503597910306No Hit
GTATTTTCACAAGAGGATTGTATGATAAAAGCAGTCAGAGGTGATCTGAA31910.1266372357127323No Hit
GTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCGAA31720.12588320641829734No Hit
GTTGCATACATGTTGGAGAGAGAACTGGTCCGCAAAACGAGATTCCTCCC31600.12540697739023318No Hit
GGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACTCTGTTCCAACAAA31180.12374017579200856No Hit
CTCCAGCACGGAGAGGGTAGTGGTGAGCATTGACCGTTTTTTGAGAATCC31140.12358143278265382No Hit
GACAATAACTTACTCATCGTCAATGATGTGGGAGATTAATGGTCCTGAAT31090.12338300402096043No Hit
CTATCAGCTGAATCAATCTCCTGGTTGCTTTTCTGAGTATGGCTGTTGCT30960.12286708924055757No Hit
CCTCTGGCTGTGACATGGTGGAATAGGAATGGACCAATAACAAATACAGT30660.12167651667039713No Hit
GCATTCTACTTTGCTTTGGTGGAGCGGCTGCGAAGGGAAGAAGTTTTATT30580.12135903065168768No Hit
TCTCATTCCTGATCCCCTCACATTCACAGTAAATGAGGAGAACTGCATTC30540.12120028764233295No Hit
TGTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCT30540.12120028764233295No Hit
TCTCTGTTCCCTGTGTTTCACTGACCTCCTCGGGAGACAGTAGTACATTT30410.1206843728619301No Hit
TGTACACACTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTTTTGCGG30300.12024782958620459No Hit
TCCCAGAACCCTACAATGCTATACAATAAAATGGAATTTGAACCATTTCA30240.12000971507217251No Hit
GAACAGATGTATACTCCAGGAGGGGAAGTGAGGAATGATGATGTTGATCA29720.11794605595056107No Hit
ATCCTGGAGTTCTTCTTTCTTCTCTTTGGTTATCGTTAGTTGCGATTCCG29520.11715234090378746No Hit
CTCCAGGATTGCAAAATTTCTCCTTTGATGGTTGCATACATGTTGGAGAG29380.1165967403710459No Hit
GTCTCGCACCCGCGAGATACTCACAAAAACCACCGTGGACCATATGGCCA29320.11635862585701383No Hit
CAATAATTGTGGCCATGGTATTTTCACAAGAGGATTGTATGATAAAAGCA29300.11627925435233645No Hit
ACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGTAT29290.11623956859999779No Hit
ACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAG29120.1155649108102402No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC29080.11540616780088546No Hit
GTGCCAAGGAGGCACAGGATGTAATCATGGAAGTTGTTTTCCCTAACGAA29040.11524742479153073No Hit
GCTTTTATCATACAATCCTCTTGTGAAAATACCATGGCCACAATTATTGC28880.11461245275411183No Hit
ACCTAAGGCCATTAGAGGCCAATACAGTGGGTTTGTAAGAACTCTGTTCC28800.11429496673540239No Hit
GGATTCAATCGCTGATTCGCCCTATTGACGAAATTCAGATCACCTCTGAC28790.1142552809830637No Hit
CCACCAATCTGTGTGCTGTGGCACATCTCCAATAAAGATGCTAGTGGATC28740.11405685222137031No Hit
GTACTACTGTCTCCCGAGGAGGTCAGTGAAACACAGGGAACAGAGAAACT28620.11358062319330614No Hit
GTGCTGGAGTACTCATCTACACCCATTTTGCTGATTCTCACTCCTCTCAT28550.11330282292693536No Hit
GAAATATCCAATTACAGCAGACAAGAGGATAACGGAAATGATTCCTGAGA28180.11183445009040414No Hit
GTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAATGTACACACTG28030.11123916380532392No Hit
CAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCCTCACATTC27950.11092167778661446No Hit
GTACATTGAAGTGTTGCATTTGACTCAAGGAACATGCTGGGAACAGATGT27820.1104057630062116No Hit
CATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAGT27680.10985016247347008No Hit
GATTGGTGGAATTAGGATGGTAGACATCCTTAGGCAGAACCCAACAGAAG27670.1098104767211314No Hit
GCTAGGAACATAGTGAGAAGAGCTGCAGTATCAGCAGATCCACTAGCATC27490.10909613317903513No Hit
CTGCTTGTTCCACCAGCCACTGGGAGGAATCTCGTTTTGCGGACCAGTTC27360.10858021839863227No Hit
GTTATAGTTGAATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTG27240.10810398937056809No Hit
GTCTGATCCTGCATCATTCATTTTACTCCATAAAGTTTGTCCTTGCTCAT27030.10727058857145579No Hit
GATTGGGATATTGCCAGACATGACTCCAAGCATCGAGATGTCAATGAGAG26850.10655624502935951No Hit
CTATGTTCCTAGCAGCAATAATCAAGCTTTGATCAACATCATCATTCCTC26840.10651655927702085No Hit
CCTCTGACTGCTTTTATCATACAATCCTCTTGTGAAAATACCATGGCCAC26770.10623875901065007No Hit
ATACAGGAGAATTGCCCCTTACAAGTATTCTCATTCCTGATCCCCTCACA26620.10564347272556986No Hit
ATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTCAATGT26620.10564347272556986No Hit
CCAATAAAGATGCTAGTGGATCTGCTGATACTGCAGCTCTTCTCACTATG26600.10556410122089248No Hit
ATACAGTTCATTATCCAAAAATCTACAAAACTTATTTTGAAAGAGTCGAA26560.10540535821153775No Hit
CTCATTGACATCTCGATGCTTGGAGTCATGTCTGGCAATATCCCAATCAT26430.10488944343113492No Hit
ACTATAACAAGGCCACGAAGAGACTCACAGTTCTCGGAAAGGATGCTGGC26390.10473070042178018No Hit
TTATTGGAGATGTGCCACAGCACACAGATTGGTGGAATTAGGATGGTAGA26320.10445290015540941No Hit
ATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTC26230.10409572838436128No Hit
CAGTAAATGAGGAGAACTGCATTCTACTTTGCTTTGGTGGAGCGGCTGCG26050.103381384842265No Hit
CATCTACACCCATTTTGCTGATTCTCACTCCTCTCATTGACATCTCGATG25940.10294484156653952No Hit
CCTATCAATGGATCATCAGAAACTGGGAAACTGTTAAAATTCAGTGGTCC25680.1019130120057338No Hit
GGAGTATACATCTGTTCCCAGCATGTTCCTTGAGTCAAATGCAACACTTC25620.10167489749170172No Hit
CCTATCGACAATGTGATGGGAATGATTGGGATATTGCCAGACATGACTCC25320.10048432492154127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA10250.0133.028121
AGCGAAA6100.089.174551
GAGCGAA2550.085.3278051
GTAGAAA12500.077.75071
CAAAAGC17750.076.3816454
GCAAAAG17900.075.753593
CGAAAGC7400.073.492713
CCGTAGA300.00193305472.528641
GCTTGCG1250.069.601234145
AGCAGGT23150.067.961528
GCAGGTC23350.067.689919
CTTGGCC4550.066.92427145
TAAACTA42550.065.945175
GAGATCG554.0629657E-665.91026145
AAGCAGG24000.065.554547
TTTAAAC42500.065.517453
AGAAACA14800.065.163533
TTGTTAA451.1918778E-464.4698941
AAACTAT43350.064.3923956
ACTATTC43150.064.017488
Produced by FastQC (version 0.11.5)