FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_DARPA_DVG_P2_NEGATIVE_CONTROL.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_DARPA_DVG_P2_NEGATIVE_CONTROL.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAAAAATACGCTGATTAAAAAAGGTGAGGTGCTATTTCGACTGGACCCGA23.8461538461538463No Hit
GACATTGAAACAATAAAAGGGACTTGAAAATTGCATTCAATTCAAACAAA23.8461538461538463No Hit
GTGTGTGTGTCGGGGGGGTGTGTATTTATTTCTGTTGGTTTAATATCTAG23.8461538461538463No Hit
TTCTTATATTTGGTTGCAGCAGGGCGGCCCTTCTTCACCAGACTGTTTCC11.9230769230769231No Hit
AACCAGGCTGGAAAATTGAGTCAATATATCGAAAGCGGTAGTCGGCGGCT11.9230769230769231No Hit
GGGTATGGAAGAAACATATCTCAATGTGATAAAAACCATACACAACATAT11.9230769230769231No Hit
GGGTTAAGGCCATGGGTACGATGAGCAATCGGCGTTGGATCCAGTTTCAT11.9230769230769231No Hit
CCATCTGATCGTTGGCAACCAGCATCGCAGTGGGAACGATGCCCTCATTC11.9230769230769231No Hit
CAGCAGTGAAGTGATTACGTGAATCAGTCAGAATCATTTTCTGATATTTT11.9230769230769231No Hit
CGCCTGGTCCATTCTGAGACTAAGAGGGCCTGCGACTTTCTGCTTGGGTT11.9230769230769231No Hit
CTGATAGACCATCGCCAAACAGATCATTCCGATCCCGGTGATCGTCCAAC11.9230769230769231No Hit
CGTCCGAAGCGGCGCCACGCATCGTCGAGCGGGCGCGAGCAGCGGACCCC11.9230769230769231No Hit
GCCTAAGGATGTCTACCATCCTAATTCCACAAAAAGTTGTGCTTTGGCAA11.9230769230769231No Hit
TTTCTCCTCTGTTTAATTTTGCTTCCTCTGAGTATTTCTGTCTCTTATAC11.9230769230769231No Hit
GCGCAGCTGCGCGTGCAAATAGACCAGCAGCGCACCGAGGCCGAACTAAG11.9230769230769231No Hit
CCCATGCCGACGGTGATCGCGGGCGAGCTGGGTGAGCTTTGCGGAGACGT11.9230769230769231No Hit
CAAAATTTCAGCCATGGTACTTTGCATTCTTGGTAAATAGTAAACATTAT11.9230769230769231No Hit
AAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATC11.9230769230769231No Hit
GGACCCTCATTGTGATGTTTTTCAAAATGAGACATGGGACCTTTTCGTTG11.9230769230769231No Hit
AAATTAAACTATTCAAAACAAAAACATGGCAATAATAATTAAAATGGTCG11.9230769230769231No Hit
GATCTTGAGCTTTCTGCGCTCGAACAAAAACAGCAGCTGGATATTATCGC11.9230769230769231No Hit
TCTTCCAGGTGCGGACGAACGGTCTGCTCCTCACGCCGACCAACGTCATC11.9230769230769231No Hit
ATACAGAGATTCGCTTGGAGAAACTGTGATGAGAATGGGAGACCTTCACT11.9230769230769231No Hit
GCATTTCTGAGCCAGTGGGGCGGATTTCTTGGCATAAATGTGATCATTGC11.9230769230769231No Hit
ACTACCACCAATCCACTAATCAGGCATGAAAACAGAATGGTGCTGGCTAG11.9230769230769231No Hit
GACCTGTTCGCCCATCTCCGGGTCGGGCACCCCCTGTCTCTTATACACAT11.9230769230769231No Hit
TCCCTCATTTGTTGGAACAGAGTTCTTACAACCCCCCTGTATTGGCCTCG11.9230769230769231No Hit
GAAGAGCCTTCGTTAAATATGCCATAACAAGTATCACCACAATTTATTCC11.9230769230769231No Hit
CACGTATCCTATCCCATATAAGGCATTACCATTCTCTACAGTCGAAAGGT11.9230769230769231No Hit
GGTGGTGGCACCGCTGCTGGTGGGGGCGCTGGGCGCGGTCTTCGAGCGCT11.9230769230769231No Hit
GAGGTGGTGTAAGGTGATAGAGGCCAGAATTTAACTATTCAACCGGACGC11.9230769230769231No Hit
CCTCTCGGCCACTCCACCACGTGGATAGGTCCGAAAGACCTCTCCGAGCG11.9230769230769231No Hit
CATCATGACAAGCACTTGCTGACCAAGCGACTGACTCAAATCTTGAGCTG11.9230769230769231No Hit
CTTTTTAGAGATAGAAGAGTTTTTAAAGAAGCTCCTTCCAGACTATGTGC11.9230769230769231No Hit
CAGTTCCTACTTCAAAAGCTCTGGCTGCTGAAGAATTAATATTATCCTTT11.9230769230769231No Hit
CATCCTGTCTCTTATACACATCTGACGCTGCCGACGAAGGCTTAGGTGTA11.9230769230769231No Hit
GAGTGCAGACCGTGCTAGAAAAGTGAGATCTTCGAACTCAGCATTCACTG11.9230769230769231No Hit
TGGGAGAGTAGGAACCAGCAAACATGACAAGATGGTATTTCCAAGATAAG11.9230769230769231No Hit
TGACTGGAGATAACACAAAATGGAGCGAAATTCAGAATACTCGGTTGGTT11.9230769230769231No Hit
GATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGC11.9230769230769231No Hit
TTGCTGGCCGCATCGCGAGCCTTCAGGTTACCACTCTCGTTATGCCTGTC11.9230769230769231No Hit
GATTATAAGAAGTGCCATAGGCCAGGTTTCAAGGCCCATGTTCTTGTATG11.9230769230769231No Hit
TCCGAGTTCTCGTCTGTCGTAGAAGTGCTTTCTGCGGATTCTACTGATAT11.9230769230769231No Hit
CTCGATGCCGAACTGCCGCTCGCCGACGCCGTGCTGATGCTGCGTGTGCA11.9230769230769231No Hit
TATGTGGTCCTTGTCGTGGTCCGTGACTATCACATAGTCTATATCCTCCA11.9230769230769231No Hit
TCCTAAAATTCCCTTAGTCAGAGGTGACAAGATTGGTCTTGTCTCTGTCT11.9230769230769231No Hit
CGTCCAGGCGGGTGCGGGTGTCGTCAAGGACTCGGTGCCCTCGGCCGAGC11.9230769230769231No Hit
TCCAAATGTAACGTTAAACAACCATAAAAAAAAATTAAGTGTTTCCTTTC11.9230769230769231No Hit
CCGATGACCCCAGCCGATCGCGTCACGATTCGTTCGAGATCGGCTACCTC11.9230769230769231No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTCGG50.0145.07
GTGTGTC50.0145.05
TGTGTCG50.0145.06
CCAGGAC50.0145.0145
TGTCGGG50.0145.08
GTCGGGG50.0145.09
GTGTGTG100.072.51
TGTGTGT100.072.52
Produced by FastQC (version 0.11.5)