FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_A549_LOW_1DPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_A549_LOW_1DPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1581587
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA51710.3269500824172177No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT47960.303239720609742No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT42850.27093040092008847No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT32060.2027077865460452No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA30990.19594242997697883No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG29420.18601569183358233No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT23090.14599260110256346No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT22770.14396931689499218No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA21930.13865819585011765No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT21140.1336632129626761No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG20960.13252511559591726No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG20640.130501831388346No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG19920.1259494419213107No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA19900.12582298665833747No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT19820.12531716560644468No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG19440.12291451560995381No Hit
AGATTATACTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTA19430.1228512879784672No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT18510.11703434588169984No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC18510.11703434588169984No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA18140.11469492351669558No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA17910.11324068799250374No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17390.10995285115520043No Hit
GTATTAAAGTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA17200.10875152615695502No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG17070.10792956694762917No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA16490.10426236432140629No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT16310.10312426695464746No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT16070.10160680379896901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA17950.0125.614534
CACGCAC3300.0118.64553
TAGTAAC20450.0110.984832
GCACGCA3550.0110.3006442
AGCACGC3700.0105.8390351
TAGAAAC8050.098.184572
GAAACAC8150.094.295784
AAACACG8200.093.723765
CGCACTT4400.088.9813165
GAGCCGC259.3896117E-486.9957145
ACGTGCA2350.083.312192
ACGCACT4900.082.8582844
CTGTCCG451.2335822E-680.55157145
GAGCAGA1200.072.519341
GTGACTT300.001935845572.5014
ACGGTAC300.001936331272.496414145
GAAGCGG2350.070.976371
ACAAGAG31550.070.777517
AGTAACA33000.069.209873
AACACGT2750.065.9099966
Produced by FastQC (version 0.11.5)