FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_A549_LOW_12HPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_A549_LOW_12HPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1805610
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT54600.30239088175187334No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA48270.2673334773289913No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT47050.2605767579931436No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG37840.20956906530203087No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA33200.18387137864765923No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT27470.15213695094732527No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27310.15125082382131247No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT26070.14438333859471314No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT25130.13917734172938784No Hit
GTGCTATACTACAAAAATCCCTGAACCAAATTGGGCTGTCTCTGGTTATT23800.13181140999440633No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT23490.1300945386877565No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG22930.12699309374671164No Hit
GCTTATATGTACCCAATCTCACCAAGGTGAGCCATTGCTTTCTCAAAATC22830.12643926429295363No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT22750.12599620072994722No Hit
AAGCAGAGCCTTTAAGATGAATATAAATCCTTATTTTCTCTTCATAGATG22450.1243347123686732No Hit
CCCATACACTGGTGTTCCCCCTTATTCTCATGGAACAGGAACAGGCTACA21820.12084558680999773No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA21070.11669186590681266No Hit
GTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCATCCTCAAA20660.11442116514640481No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA20350.112704293839755No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG20340.11264891089437917No Hit
TCTGCATACTGTCCAATCAAAAGTCTGTCTCCCCTGGGTTAATTTGTCTA19560.1083290411550667No Hit
GTATTAAAGTATAATCTAATGGACCCTGAATACAAAGGGCGGTTACTTCA19400.1074429140290539No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG19390.1073875310836781No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG19370.1072767651929265No Hit
CTTTTAGGTTGAATGATTTAAATGGAGCCGACAAAGGTGGATTAATACCT18890.10461838381488804No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT18670.10339995901662041No Hit
ACCTAAAAGAGGTTATTGTTGTGTTCAGTGCCGTTGCAGCAGGTTGGTTT18430.1020707683276012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA16100.0128.33884
CACGCAC5700.0115.771533
GCACGCA5850.0111.581972
AGCACGC5900.0110.670091
TAGAAAC8800.0108.792432
GAAACAC8850.0104.039784
CGCACTT6500.0102.6241465
TAGTAAC20400.0102.0373462
AAACACG9100.0101.1899645
ACGCACT7250.093.000214
AACACGT2800.082.861926
CGGTGCG1200.072.528292
ACGTGCA3550.069.4637152
ACAAGAG30900.067.342077
AACACGA10150.067.1467366
ACACGTG3500.066.2895367
GAAGCGG2050.063.702781
GGTGCGT1400.062.1567733
CACGTGC6400.061.2143971
AGTAACA34750.060.308463
Produced by FastQC (version 0.11.5)