FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_A549_HIGH_6HPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_A549_HIGH_6HPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1336211
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA37480.2804946224810303No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT36320.27181335881833035No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC31340.23454379585260113No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT27560.20625485046897535No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA26550.19869616400403828No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT23830.17834009748460386No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG23660.1770678433271392No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT23650.17699300484728833No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT20930.15663693832785391No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA20910.15648726136815216No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG17440.13051830885990312No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG17230.12894670078303502No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17220.12887186230318415No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17150.12834799294422813No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA15380.11510158201062558No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT14880.11135965801808247No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT14670.10978804994121437No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT14280.10686934922703076No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT14140.10582161050911869No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTG100.0070870733145.027732
TATCGAG100.0070870733145.027732
CACGCAC4650.0123.181763
GTAACAA15750.0119.222724
AGCACGC4800.0117.852671
GCACGCA4800.0117.835032
CGCACTT5750.099.612835
TAGAAAC6000.096.685162
AAACACG5700.096.6706855
GAAACAC5650.096.2393344
AACACGT1450.095.003956
ACGCACT6150.094.309334
TAGTAAC21550.088.8336942
ACACGTG1700.081.029757
CGGTGCA702.8103386E-972.513862
ACGTGCA1750.070.442042
CACGTGC3500.066.308321
TAACAAG29150.064.170765
AGTAACA30300.063.4125983
GAAGCGG2000.061.646021
Produced by FastQC (version 0.11.5)