FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n02_A549_HIGH_6HPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n02_A549_HIGH_6HPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1775660
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT50910.28671029363729544No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA50400.28383812216302673No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC42420.2388970861538808No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT38590.21732764155300002No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA37940.21366703085050065No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG32370.18229841298446775No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT30060.16928916571866237No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT29270.1648401157879324No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA27950.15740626020747214No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT27320.15385828368043433No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA25060.14113062185328273No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG24380.1373010598875911No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG22910.12902244799116946No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT22470.12654449613101607No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG21430.1206875190070171No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA21240.11961749434013268No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT18950.1067208812497888No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT18700.10531295405651983No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT18690.10525663696878908No Hit
CCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGAAAAAGCTAAA17990.10131444082763592No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCCG100.0070921914144.99406145
AGCACGC6400.0122.380081
GCACGCA6350.0122.1913152
CACGCAC6450.0120.279933
GTAACAA19350.0116.894434
GAAACAC8400.0110.4716644
AAACACG8900.0104.27425
ACGCACT7600.0102.0681844
CGCACTT7800.099.459455
TAGAAAC9750.098.918452
TAGTAAC26700.086.366662
GAAGCGG1600.077.054131
AACACGA9500.074.029546
AACACGT2950.073.7320256
CTGTCCG406.665963E-572.49703145
ACACGAG10300.068.279677
ACACGTG3200.067.97177
ACGTGCA3100.065.497792
CGTGCTC451.1943546E-464.4418145
AACGGCC702.3362372E-762.140312145
Produced by FastQC (version 0.11.5)