FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12743968
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5994454.703754748913369No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG213470.16750669807080495No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA184420.1447116000291275No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA184150.14449973509035804No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTAAC28800.078.958052
GCACGCA11400.067.3299942
CGCACTT13500.064.2945865
ACGCACT12800.063.887774
GAGCGAA8700.060.2330861
GCAGGTC61900.059.196549
CTATTCG68850.058.532689
GCAAAAG81450.057.5102123
CACGCAC14700.053.6782263
TAAACTA82200.050.091375
GAGCAAA93700.049.56741
TTTAAAC92000.044.053813
AAACACG18000.042.6416635
TTTTTAA102250.038.1915321
CACGAGC16250.034.4199878
TAACAAG78300.032.7977565
CTAGTAG7150.032.1045653
CAGGTCA61950.030.3269988
CGAAAGC33650.028.9919013
ACAAGGT48800.028.947827
Produced by FastQC (version 0.11.5)