FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_INFANT_MICE_Exp2_Mother_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_INFANT_MICE_Exp2_Mother_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences883508
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG14960.16932500894162816No Hit
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA14590.16513715778464938No Hit
ATAATACACAATGCCATCTACCAAATATTACCCTTCCCCAAAGCAGCACA12600.1426133096700879No Hit
TACATACACTCATACACAATAGCTCCCAAAAAAGACCTAAGTACTCGGCG10300.1165807213969766No Hit
GTGTATTATATGGCATGAGTAGGGTAGCAGTGCATCTTGAAGTATGTAAA10210.1155620548993331No Hit
GTTATTATACAGGGAAACCATCCATTCCAGGCCCTGGAGCTGGTAATGCT9490.10741272291818524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2100.065.61881
CCGTACG350.003565291462.147587145
CGCCGGA350.003565291462.1475872
GAGCAAA21300.057.8846361
GCAAAAG21400.056.920223
AGCAAAA24450.050.4128342
ACGGTCC604.945672E-448.331548
AGCGAAA4100.045.9921261
AGCAGGC14750.037.8460548
CGAAAGC5250.037.288553
CAAAAGC38500.032.203754
AAGCAGG45400.030.6596557
TATACTA1555.4968546E-528.0666525
TAGTACC2104.98203E-727.6211514
AAAAGCA48750.026.0276225
GCGAAAG7900.024.7803652
GTACCCG2351.3292647E-624.6827286
TGTATTA8300.024.4596922
TATATGG7750.024.321687
TTATATG8650.024.3082086
Produced by FastQC (version 0.11.5)