FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_INFANT_MICE_Exp1_Mother_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_INFANT_MICE_Exp1_Mother_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences960657
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATACACAATGCCATCTACCAAATATTACCCTTCCCCAAAGCAGCACA16770.1745680300044657No Hit
GAGCAAAAGCAGGCCCAAGCAGCCAAAGAGAAGAAGAAGAGGAGGAGGAG16490.17165335806640664No Hit
GTACTGCATGCTGTATTCGTCGTCCACGTCCTGCTTCGCAGTCTCAATGA15490.1612438154304814No Hit
GTGTATTATATGGCATGAGTAGGGTAGCAGTGCATCTTGAAGTATGTAAA12340.12845375612731705No Hit
TACATACACTCATACACAATAGCTCCCAAAAAAGACCTAAGTACTCGGCG12210.12710051558464677No Hit
ATATAATACACAATGCCATCTACCAAATATTACCCTTCCCCAAAGCAGCA10570.11002886566172942No Hit
GTTATTATACAGGGAAACCATCCATTCCAGGCCCTGGAGCTGGTAATGCT10230.10648962116551486No Hit
GTACAGGACCAACTGCCAGAAAAGTTATTGATAATTTTATTCCTTAAGAA9870.10274218581658179No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA2450.073.998081
GCAAAAG23350.062.1107833
GAGCAAA26250.058.567011
AGCGAAA5050.056.0040931
AGCAAAA29900.049.2321432
TACGTAG450.00963149348.3428965
CGAAAGC6650.046.8889733
TCTATAC1255.187758E-946.409183
AGCAGGC19050.038.437928
CAAAAGC42350.034.2452624
AAGCAGG50200.032.4946827
GCAGGCC21700.027.7301279
AAAAGCA52750.027.6310585
TTCGCTC1100.00966207926.368854
GCGAAAG12200.025.5583342
TAATACA11950.025.4862122
GTATAAG6550.025.4643761
ATAATAC11250.025.1396121
GGGTAAT6350.025.1243881
CCTACTT7050.024.683163145
Produced by FastQC (version 0.11.5)