FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_INFANT_Exp2_Shed_Ind2_D2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_INFANT_Exp2_Shed_Ind2_D2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1127322
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT24590.21812756248880089No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG22260.19745911106143588No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC21340.18929817745063077No Hit
CATATGGAGCATGCCCCAAGTATGTTAAGCAAAACACCCTGAAGTTGGCA21150.1876127672483993No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT20500.18184689024076528No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT20420.1811372438398257No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA19650.17430689723078235No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC19470.17271019282866829No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC18440.1635734954165713No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT17600.1561222082067058No Hit
GTCTAGTAGAATAAGCATCTATTGGACAATAGTTAAGCCGGGAGACGTAC17070.15142080080048115No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT17000.15079986019965902No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT16870.14964668479813223No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA16090.14272763238897138No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA15990.14184057438779693No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA15690.1391794003842735No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA15670.13900198878403863No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA15110.13403446397746163No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA15020.13323611177640463No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT14880.13199423057476037No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT14550.12906693917088463No Hit
ATATTCGGGATTATAGTTTGCTGGCTTCTCCTGGTAGAGACTGTGACTCT14370.1274702347687706No Hit
CTATTGGGGGACCCTCATTGTGATGTTTTTCAAAATGAGACATGGGACCT13970.12392200276407273No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT13800.12241400416207614No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA13770.1221478867617238No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT13390.11877706635726085No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC12830.11380954155068383No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA12560.11141448494751277No Hit
CTCCATATGTGATCTTGTTTACGTTTTGAAAGGGCTTGTCATTGGGAATG12430.11026130954598598No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT12310.10919683994457662No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA12270.10884201674410683No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA11480.10183425853482857No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA11320.10041496573294942No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGGGC100.0070911227144.999043
ATATGCG100.0070911227144.999046
CGCACGA100.0070911227144.99904145
GAGCGAA1700.0132.234311
TGTAGCT256.4654487E-6115.99922145
CTACTAA301.5996018E-596.6660166
ACACCGC301.5996018E-596.666016145
AGCGAAA3500.076.659351
AGTGCCG406.663776E-572.49952145
GGCAGCC1100.072.49951145
CACTTCG300.001935858372.49951145
GCAGGAG702.8139766E-972.49639
ACAAGGG3650.069.520083
AGCAGGC1650.065.905728
CAAGGGT4050.062.65394
CGAAAGC4750.062.5785263
AAGGGTG3500.060.0710265
GCAGGGT9800.056.221629
CAAAAGC22800.055.0105974
AGGGTGA7050.051.4158139
Produced by FastQC (version 0.11.5)