FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_INFANT_Exp2_Shed_Ind1_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_INFANT_Exp2_Shed_Ind1_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1365953
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC154381.1301999409935775No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT135020.9884673923626948No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA119470.8746274578993567No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA88710.6494366936490494No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA72440.5303257139886951No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA69860.51143780203272No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG67540.4944533230645564No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA62210.45543294681442187No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA60600.44364630408220485No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC57940.42417272043767246No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT46230.33844502702508794No Hit
GCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGA44240.3238764437722235No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA35660.26106315517444595No Hit
GAATAAAAGAACTAAGAAATCTAATGTCGCAGTCTCGCACCCGCGAGATA35570.26040427452481896No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC35320.25857405049807713No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG35160.2574027071209624No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA34910.25557248309422065No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA31230.22863158542058182No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA30990.22687457035490974No Hit
GTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGTCTCCTTGC30970.2267281524327704No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC30540.22358016710677456No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG30290.22174994308003276No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG29910.21896800255938528No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT29490.2158932261944591No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT28730.2103293451531641No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA27430.2008121802141069No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT26570.19451620956211524No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG26150.19144143319718906No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT25380.18580434319482442No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT25030.18324202955738594No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT24790.18148501449171384No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG24250.1775317305939516No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG24160.1768728499443246No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT23890.17489620799544348No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA23850.17460337215116478No Hit
GGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATG23470.1718214316305173No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCC23470.1718214316305173No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC22980.16823419253810343No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG22930.16786814773275507No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTGAACTCTTCTTTCTTT22710.1662575505892223No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA22520.1648665803288986No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC21540.15769210214407084No Hit
CCATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACC21250.1555690422730504No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA21210.1552762064287717No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA20980.1535924003241693No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA20710.15161575837528818No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA19370.1418057575919523No Hit
CTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAAC19330.1415129217476736No Hit
TTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCT19230.1407808321369769No Hit
ACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCC19150.14019516044841954No Hit
ATCTAATGTCGCAGTCTCGCACCCGCGAGATACTCACAAAAACCACCGTG19060.1395362797987925No Hit
AAACAAGGTCGTTTTTAAACTATTCGACACTAATTGATGGCCATCCGAAT18930.13858456330488678No Hit
CAATTAGCACATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTG18520.13558299590103026No Hit
GTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCACT18470.13521695109568194No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA18290.13389918979642784No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT18170.1330206822635918No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG17740.12987269693759596No Hit
ATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAG17680.12943344317117791No Hit
CTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGG17630.12906739836582956No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA17080.12504090550699767No Hit
AGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT17000.12445523381844031No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC16970.1242356069352313No Hit
GATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGATTGTC16770.12277142771383788No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT16540.12108762160923546No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG16420.12020911407639942No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC16210.1186717258939363No Hit
GAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCATTAATCAGGCA16130.11808605420537896No Hit
ATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGC16050.11750038251682159No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT15890.11632903913970687No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT15880.11625583017863719No Hit
AGCGAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG15680.11479165095724378No Hit
GAGCGAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT15670.1147184419961741No Hit
CAATTAGCCTTCTCTCCTTTCGCAAGGTTGCTCAGTTCATTGATGCTTAG15290.1119365014755266No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT15040.11010627744878485No Hit
GGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGA14900.10908135199380944No Hit
CAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTG14450.10578694874567426No Hit
AGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGT14410.10549411290139557No Hit
ATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCAGTC14330.10490844121283822No Hit
GCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTTTC14280.10454239640748987No Hit
GCTAAATTCACTATTTTTGCCGTCTGAGCTCTTCAATGGTGGAACAGATC14260.10439597848535052No Hit
ATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCC14180.10381030679679315No Hit
ATATGGCCATAATCAAGAAGTACACATCAGGAAGACAGGAGAAGAACCCA14050.10285859030288744No Hit
GTCAGTAAGTATGCTAGAGTCCCGTTTCCGTTTCATTACCAACACCACGT13870.10154082900363337No Hit
CAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCA13730.10051590354865797No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT13720.1004426945875883No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTT13680.1001498587433096No Hit
AGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAAG13660.10000344082117028No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTA100.0070898747145.00868145
GAGCGAA4450.0136.872131
TTAGTAG900.096.6795351
GGCCCGG451.0915755E-896.672455145
CAAGTTA301.5991951E-596.67245145
GAGCAAA50950.089.516331
GCTTTCG651.6370905E-1189.2426451
AGGCCGC259.385258E-487.00521145
AGCAGGC9400.080.982968
GCAAAAG57300.078.575123
AGCGAAA9800.074.7293241
CAAAAGC60650.074.235034
TGTCGTG300.001935435372.50433145
TTTAAAC13350.070.600893
GGCAGCC1750.070.432785145
TAAACTA13500.069.816445
TTTTAAA14250.065.63312
AAGCAGG77950.065.577
TTTCGCT902.8194336E-1064.445943
CGCTCCC902.8194336E-1064.445946
Produced by FastQC (version 0.11.5)