FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_INFANT_Exp2_Shed_Ind1_D1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_INFANT_Exp2_Shed_Ind1_D1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1493062
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG35120.23522131030057694No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT32710.21907998462220593No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC30620.2050819055069381No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT27670.1853238512533304No Hit
CATATGGAGCATGCCCCAAGTATGTTAAGCAAAACACCCTGAAGTTGGCA27410.18358246342080906No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA25520.17092391340748075No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT24780.16596765573030456No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC24740.16569974990991665No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT24540.16436022080797716No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT24080.1612793038735163No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT23530.15759559884318267No Hit
GTCTAGTAGAATAAGCATCTATTGGACAATAGTTAAGCCGGGAGACGTAC23180.15525142291478855No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT22560.15109888269877608No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT21980.1472142483031515No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA20930.1401817205179691No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA20220.13542639220608388No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA19640.13154175781045932No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA19350.12959944061264703No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA19040.1275231705046408No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT18870.12638457076799223No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA18680.1251120181211497No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA18640.12484411230076178No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC17950.1202227368990705No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG17410.11660600832383383No Hit
CTCCATATGTGATCTTGTTTACGTTTTGAAAGGGCTTGTCATTGGGAATG16760.11225253874253047No Hit
CTATTGGGGGACCCTCATTGTGATGTTTTTCAAAATGAGACATGGGACCT16650.11151579773646372No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA16350.10950650408355447No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA15640.10475117577166922No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT15610.1045502464063783No Hit
GTTCTTATCTCTTGCTCCACTTCAAGCAATAGATGTAAGGCTTGCATAAA15210.1018711882024993No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGGGC100.007089761145.0099145
GGTCCGA203.8643533E-4108.764721
CTACTAA553.6379788E-12105.45476
GAGCGAA1600.099.700991
TTAGTAG451.0910298E-896.679751
AGCGTAC301.5991758E-596.67328145
AGCGAAA3500.084.940061
GCGCAAA406.659795E-572.509811
CTTTATA406.659795E-572.509811
ATAGGGG300.001935919272.500113
AGCAGGC1200.072.497678
GCAGGGT13700.069.8517769
ACACCGC451.1936466E-464.44885145
TTTATAT451.1940423E-464.4445342
AAGGTCG1250.063.7979559
TTTAAAC2100.062.1429443
CAAAAGC27750.061.657744
AAGGGTG7350.061.1565485
ACAAGGG8250.058.8788643
CAAGGGT8350.058.1737334
Produced by FastQC (version 0.11.5)