FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_INFANT_Exp2_Shed_Cnt5_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_INFANT_Exp2_Shed_Cnt5_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences840399
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA3200.081.57841
GCGAAAG3400.078.898352
CGAAAGC3700.072.501193
AGACGCG604.9427775E-448.337145
GGTACTG4600.031.5222555
CTAACCG2405.0767994E-827.1879466
TAATGGT3657.2759576E-1225.8223424
GTACACT5000.023.200384
GTATTAC3803.619789E-1022.89921
TAACCGA2852.778852E-722.8937517
TCTATGC7700.022.5977733
TATGCTG9050.022.4313075
GTATATA5850.022.3120421
CATAGAC7450.021.4097463
CCAGACG1700.003092094321.323884
TATAGGA10700.021.0050182
GTACTGA7650.020.8500166
GTCCTAG3508.7140506E-820.7183251
ATAGGAT2806.0874954E-620.7146263
CGTACAC1800.004306100320.139224
Produced by FastQC (version 0.11.5)