FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_INFANT_Exp2_NL_Cnt5_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_INFANT_Exp2_NL_Cnt5_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1827989
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC171470.9380253382268712No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT132080.7225426411209258No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA115600.6323889257539296No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA97310.5323336190753882No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA93100.5093028459142807No Hit
GTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGCA80980.4430004775739898No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG69500.3801992243935822No Hit
ATACCGACTTTGCTAATATTTCAAATCTGTGCTTCAAAAGTGCATTTGGA63320.34639158113095864No Hit
GCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTC53990.2953518866907842No Hit
GTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGAT53200.29103019766530325No Hit
GTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAA52220.28566911507673187No Hit
AGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATGC51620.2823868196143412No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA50710.2774086714963821No Hit
AATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCA44010.2407563721663533No Hit
GAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGCAATATG42340.2316206497960327No Hit
GAGTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAGGCAATG32930.18014331596087285No Hit
GCCTAATACAGTGCTTAACATATTGAACATGCCCATCATCATTCCAGGGC31160.17046054434682048No Hit
ATAAGAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGA31130.17029642957370092No Hit
CTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAA30270.16559180607760768No Hit
AGTCAGTATTCAACAGCTTGTATGCATCTCCACAACTAGAAGGATTTTCA29830.16318478940518788No Hit
GAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAAGGCGA29360.16061365795964855No Hit
TCGTAGAACTTGGTGATCCAAATGCACTTTTGAAGCACAGATTTGAAATA29350.16055895303527537No Hit
ATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCTAAGAG28910.15815193636285557No Hit
CATATATGACAAGAAATCAGCCCGAATGGTTCAGAAATGTTCTAAGTATT28320.1549243458248381No Hit
GCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAGAGTAT28250.15454141135422586No Hit
ATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCAGC27600.15098559126996935No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAAAGAATAAAAGAACTAA27320.1494538533875204No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA27110.14830504997568367No Hit
CCTCAATTGCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGG27020.14781270565632507No Hit
GAAGCAGTTTTCTTGATTCAGCTGAAAATCCTTCTAGTTGTGGAGATGCA26730.1462262628495029No Hit
GCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCA26650.14578862345451749No Hit
GTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGA26460.14474922989142713No Hit
CCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAAAT26250.14360042647959043No Hit
ATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTGAA26130.14294396738711226No Hit
TCATTAATCAGGCACTCCTCAATTGCTTCATATAGCCCCCCAAGATCAAA25660.14037283594157296No Hit
CAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAA25600.1400446063953339No Hit
GCTTCATATAGCCCCCCAAGATCAAAGGTCCCAGGTTCAAGGTTGTCCCT25210.13791111434477998No Hit
CTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAAG25150.13758288479854092No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC24230.1325500317562086No Hit
AACTAACTCAATGCATGTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAG24020.13140122834437187No Hit
GGTTCAAGGTTGTCCCTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGA24010.1313465234199987No Hit
TTTTTAAACTATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG23000.1258213260583078No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA22990.1257666211339346No Hit
CAATGAAAGAGTATGGGGAGGACCTGAAAATCGAAACAAACAAATTTGCA22810.12478193249521742No Hit
GTTCAGAAATGTTCTAAGTATTGCTCCAATAATGTTCTCAAACAAAATGG22650.12390665370524659No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA22580.12352371923463434No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCT22030.12051494839410959No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG21810.11931144005789969No Hit
GCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAG21580.11805322679731663No Hit
GCAATATGCACTCACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTT21540.1178344070998239No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC21510.11767029232670437No Hit
AGTAGAAACAAGGTACTTTTTTGGACAGTATGGATAGCAAATAGTAGCAC21400.11706853815859943No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT21000.11488034118367232No Hit
GCATTAAGCAAAACCCAGGGATCATTAATCAGGCACTCCTCAATTGCTTC20530.112309209738133No Hit
CTAAGAGCCTGAACGATAAGAAGCAGTTTTCTTGATTCAGCTGAAAATCC20090.10990219306571321No Hit
GTATACATCATGGTCATAAGTCCCATTTCTGATTGACTCTATGCAAGCGT19840.10853456995638376No Hit
GTGTAAGGAAGGAGTTGAACCAAGAAGCATTAAGCAAAACCCAGGGATCA19750.10804222563702517No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC19420.1062369631327103No Hit
ACAATGCTTCAATCCGATGATTGTCGAGCTTGCGGAAAAAACAATGAAAG19350.10585402866209809No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT19260.10536168434273949No Hit
ATATTGAACATGCCCATCATCATTCCAGGGCTCAATGATGCAGTCCCCTC19090.10443170062839546No Hit
CCTCTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCA19040.10415817600652959No Hit
GTATATGTGAGAAACTTGAACAATCAGGGTTGCCAGTTGGAGGCAATGAG18980.1038299464602905No Hit
ATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGAACCCA18870.10322819229218556No Hit
GATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCCGATGATTGTC18700.10229820857784155No Hit
GTTCTAAGTATTGCTCCAATAATGTTCTCAAACAAAATGGCGAGACTGGG18600.10175115933410979No Hit
ATTATATTCAATATGGAAAGAATAAAAGAACTAAGAAATCTAATGTCGCA18560.10153233963661706No Hit
AGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTT18390.10060235592227307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAGA100.0070895157145.012481
GGCGACC151.2300171E-4145.00455145
CTAGAGC100.0070906756145.004533
GCTATGC100.0070906756145.00453145
CGACTAA100.0070906756145.004535
GAGCGAA2400.0129.907011
TTAGTAG1150.081.963581
AGCGAAA5950.080.4270861
AGCAGGC2350.080.2108848
CTACTAA554.395588E-879.093386
GCAAAAG48600.074.292463
GGCTTTA300.001935758972.50227145
GGGGGCA300.001935758972.502274
GCTTTCG406.665435E-572.49837
CAAAAGC53700.066.966714
GAGCAAA54400.066.775051
GCAGGGT11450.066.483169
GGGGAGA554.060874E-665.914761
TTTAAAC6350.062.797243
GTATAGA350.003565760662.1482051
Produced by FastQC (version 0.11.5)