FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_INFANT_Exp2_NL_Cnt2_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_INFANT_Exp2_NL_Cnt2_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1241525
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC25680.20684239141378546No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT25230.20321781679788967No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA24540.19766013572018287No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG23940.19282736956565513No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC21870.17615432633253458No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA20570.16568333299772459No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA20410.16439459535651718No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC20310.16358913433076255No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT20260.16318640381788527No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC19630.1581119993556312No Hit
GTATAGGAAGCTCAAGAGGGAGATAACATTCCATGGGGCCAAAGAAATCT19310.1555345240732164No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA16260.1309679627877006No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA16240.1308068705825497No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT15850.1276655725821067No Hit
CATATGGAGCATGCCCCAAGTATGTTAAGCAAAACACCCTGAAGTTGGCA15470.12460482068423913No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT15360.12371881355590907No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT15320.12339662914560721No Hit
CCGCTATATGATGCAATCAAATGCATGAGAACATTCTTTGGATGGAAGGA15270.1229938986327299No Hit
GAGCAAAAGCAGGTAGATATTGAAAGATGAGTCTTCTAACCGAGGTCGAA15000.12081915386319243No Hit
ATATACAACAGGATGGGGGCTGTGACCACTGAAGTGGCATTTGGCCTGGT14690.11832222468335313No Hit
GTTGTATATGAGGCCCATACAACTGGCAAGTGCACCAGCAGAATAACTGA14380.11582529550351382No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT14030.11300618191337267No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG13850.11155635206701435No Hit
GAACTAGGTGATGCCCCATTCCTTGATCGGCTTCGCCGAGATCAGAAATC13630.1097843378103542No Hit
GCTCTATGCTGACAAAATGACCATCGTCAGCATCCACAGCACTCTGCTGT13550.10913996898975051No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT13430.10817341575884497No Hit
CTACTAGACAGACCCCTTACCCAGGGTCTGGACCCGATATTCGGGATTAT13340.1074485008356658No Hit
CTCATAGACTTTGGCACTCCTTCCGTAGAAGGCCCTCCTTTCAGTCCGTA13270.10688467811763758No Hit
TCTCTGTACTGAGAACGTAGGTTGTATGCTGATTTGGCCCGCAGATGCCC13030.1049515716558265No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG12890.10382392621977005No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCACAGAGACTTGAA12570.10124645093735526No Hit
GGTATGTGCAACCTGTGAACAGATTGCTGACTCCCAGCATCGGTCTCATA12480.10052153601417611No Hit
GTATTGGTTCATGGTCTCAAAATATCCTCAGAACCCAGGAGTCGGAATGC12470.10044098991160064No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1200.0102.721571
CTACTAA451.0915755E-896.671346
AGCGAAA2950.090.9439241
TAAACTA1150.088.265145
GGTCGTT353.4381534E-582.854487
TTTAAAC1250.081.2039263
ATTAGTA554.3914042E-879.1011051
GGCAGCC1050.075.95299145
AGGTCGT406.662223E-572.50356
TAGGCCG300.001935790972.50059145
CGAAAGC4050.068.027983
GCAGGGT9250.065.0519569
GCAAAAG23550.062.8055843
AGCTTAT1051.6370905E-1162.1508671
TTAGTAG702.3342182E-762.1458632
CAAAAGC24200.061.118664
AAGGTCG606.8046083E-660.4195865
CAAGGTA2450.059.1817679
TGAAGCG502.0103544E-458.000465145
GCCCACC400.006051034754.375443145
Produced by FastQC (version 0.11.5)