FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_INFANT_Exp1_NL_Ind2_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_INFANT_Exp1_NL_Ind2_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1183348
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACCTTACTTT35060.29627801796259423No Hit
CAGTATCCTTCCAGATTCGAAATCAATCCGTGCATCAATTCGGGCTCTGG31460.2658558598146953No Hit
AGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCTTCAATCC28410.2400815313838364No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAA27280.23053235396519028No Hit
CCCTTATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACA26980.22799717411953205No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGT24320.2055185794880289No Hit
GAGCAAAAGCAGGTACTGATCCAAAATGGAAGATTTTGTGCGACAATGCT22900.19351872821857982No Hit
GAGCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAG22120.18692726061986836No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTA21980.18574417669189452No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCC21920.18523714072276287No Hit
CCTTACAGCCATGGGACAGGAACAGGATACACCATGGATACTGTCAACAG21820.18439208077421013No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCA21250.17957523906745945No Hit
GGATACACCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGG20910.1767020352423801No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGCTAAATTCACTATTTT20760.17543444531955096No Hit
CAGTGAACTCTTCTTTCTTTATCCTTCCAGATTCGAAATCAATCCGTGCA19710.1665613158597471No Hit
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATC18720.15819522236907485No Hit
CCTATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTA18410.15557553652856135No Hit
ATACTGGATATCCCGACTGGTCTTCTGTATGAACTGCTGGGGAAGAATTT17820.1505896828321001No Hit
CTCTAATATTGCTAAGGGCTTTCACCGAAGAGGGAGCAATTGTTGGCGAA17580.1485615389555735No Hit
CTATTAAGAGCGGTCGGATTTTTTCAATCTTCTTTCTTGTTGAATCATTG17480.14771647900702076No Hit
ATATACAGGAGAGTAAACGGAAAGTGGATGAGAGAACTCATCCTTTATGA17470.1476319730121655No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAA15460.1306462680462552No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTT15460.1306462680462552No Hit
ATACTGGAGACCCTCCTTACAGCCATGGGACAGGAACAGGATACACCATG15050.12718152225718893No Hit
CATCAGTACTCAGAAAAGGGAAGATGGACAACAAACACCGAAACTGGAGC14910.12599843832921506No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCT14740.1245618364166754No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGTATCCTTCCAGATTCGA14600.12337875248870155No Hit
GTATATAGGTCCTCCAGTTTTCTTAGGATCTTTCCCCGCACTGGGATGTT14580.123209740498991No Hit
GCAAAAGCAGGGTGACAAAGACATAATGGATCCAAACACTGTGTCAAGCT14520.12270270452985936No Hit
GTATTAGGCGTCTCCATCCTGAATCTTGGACAAAAGAGATACACCAAGAC14100.1191534527459378No Hit
CCATGGATACTGTCAACAGGACACATCAGTACTCAGAAAAGGGAAGATGG12650.10690008349192291No Hit
GACATAATGGATCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCT12630.10673107150221237No Hit
AAACAAGGGTGTTTTTTATTATTAAATAAGCTGAAACGAGAAAGTTCTTA12580.106308541527936No Hit
GAGCAAAAGCAGGGTAGATAATCACTCACTGAGTGACATCAAAATCATGG12300.10394237367198829No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATCG100.0070897085145.0096
GAGCGAA2200.0128.536131
GTTGACG203.8659287E-4108.75216145
AGCAGGC6650.085.035688
TTTAAAC2150.074.190653
TAAACTA2150.074.190655
GGCAGCC1000.072.50144145
GCAAAAG25300.070.211863
AGCGAAA4250.069.948471
TGGGCCC2000.068.876366145
CAAAAGC26800.066.28214
CCATCGG350.003565191362.149341
CTATGCT4250.061.4155774
ATTAGTA606.8021545E-660.422971
TTAGTAG606.803859E-660.4204142
GCAGGGT9600.059.6601229
GAGCAAA31250.059.1661761
TTTTAAA2850.055.9683882
ACAAGGG4700.055.5353623
GCAGGAG1051.1095835E-955.2368559
Produced by FastQC (version 0.11.5)