FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_HBEpC_LOW_3DPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_HBEpC_LOW_3DPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1376083
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA37860.27512875313480367No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT37100.26960583046226133No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT36520.2653909684226896No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC24370.17709687569717814No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA24360.1770242056620131No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG23550.1711379328136457No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT22850.1660510303520936No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT22300.16205417841801695No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA21020.1527524139168931No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG21000.15260707384656302No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATATATCACCCAGG20770.15093566303776734No Hit
ATACAGAAGATTGGAATATGGGACGGGGAAGAGGAGTTCCATGTAAGATG20310.14759284142017595No Hit
CTTTAATACTATTTCTGGGATATGCAAGTTTCTCAAATTGTAAATGTTGG18200.13225946400035463No Hit
GTATAGTCAGATGCATTCATTGATTCATTTATCCCATGTAGTTCACTTGC17910.13015203298056877No Hit
GTTCTACAGAGATGGATTTGTATCTAATTTTGCAATGGAACTCCCTTCGT17760.1290619824530933No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17470.12695455143330744No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA17320.12586450090583198No Hit
GTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATCCTCTT16360.1188881775299891No Hit
GGATGAAGAAACATGTATGGAAGGAATAAACGACTTTTACCGAACATGTA16250.11808880714317378No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT15940.11583603605305785No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT15720.11423729527942719No Hit
TCTTCATCCTTTGCATTAACAAATAGAGCAAAATCATCAGAAGATTGCAG15410.11198452418931125No Hit
CTTCTGTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATC15350.11154850397832108No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT15350.11154850397832108No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT15200.11045845345084562No Hit
GAACAATACAGAAGATTGGAATATGGGACGGGGAAGAGGAGTTCCATGTA15040.10929573288820514No Hit
GATATATCCCTTAGTCTAGATATTGTTTGTCCAAGTTTAAAATCAATTCC14870.10806034229039964No Hit
GTCCTGATCTGTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGG14510.10544422102445855No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA14400.10464485063764323No Hit
GTACAGTGGATTTGCAAGAGCAGTGCTCAAACAAATGAGAGACCAAGAGG14330.10413616039148801No Hit
GAATAGAGACTCCCAAGTATCTGGCTGATTTGTTTGATTATAAAACCAAA14280.10377281021566286No Hit
GTATTTTCTCAAGACACTAGGATGTTCCAAGGGGTGAGAGGAGAAATAAA14280.10377281021566286No Hit
CATTTATCCCATGTAGTTCACTTGCTTTGGGTGATTCCTCATACCCCCAT14080.10231940951236226No Hit
TCATTGGACAAAGCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTT13840.10057532866840153No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCACGC2950.0130.281891
GCACGCA2950.0130.262952
GTAACAA14250.0128.723244
CACGCAC3100.0123.95543
CTCCGAC256.4635933E-6116.00776145
GAAACAC6750.0111.7071154
CTGTCCG203.8653405E-4108.75728145
AGTCGTA203.8653405E-4108.75728145
AAACACG6900.0108.227945
TAGTAAC17550.0103.2832642
TAGAAAC7100.0103.140692
CGCACTT4050.0100.2499855
ACGCACT4150.096.087284
AACACGT2300.088.2635656
GACGATT259.386363E-487.002664
CGGTGCG703.092282E-1182.8626862
CCACGAC353.436597E-582.862686145
ACACGTG2450.082.856677
ACAAGAG22150.082.155287
GGTGCGT755.638867E-1177.33573
Produced by FastQC (version 0.11.5)