FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_HBEpC_LOW_2DPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_HBEpC_LOW_2DPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences934272
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT20820.22284730785039047No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA16910.18099654062200302No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT16450.17607292094807508No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT14540.15562919578024387No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT14480.15498698451842718No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG14320.15327442115358267No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC14060.15049150568571038No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA13740.14706637895602137No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT13300.142356829702699No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT13250.1418216536511851No Hit
ATGCTACACTGCTTACAAGGTGAAAATTGGAGACAAATATATCACCCAGG12050.12897742841485135No Hit
GTTTAATATGCTATCTACCGTGTTGGGAGTAGCTGCACTAGGTATCAAGA12000.12844225236333745No Hit
ATACAGAAGATTGGAATATGGGACGGGGAAGAGGAGTTCCATGTAAGATG11680.12501712563364845No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA11590.12405380874092342No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT11310.12105682285244554No Hit
GTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATCCTCTT11310.12105682285244554No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG11290.12084275243183999No Hit
CTTCTGTATTGTTCCGTTCCCTATTAATATTTCTTCATCATTTTTGAATC10790.11549099191670092No Hit
CTATTTTCCTATAATCCACAAACAGAAGTCCTAACTATATGCGGCAGAAT10600.11345732292094807No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT10200.10917591450883683No Hit
GTATAGTCAGATGCATTCATTGATTCATTTATCCCATGTAGTTCACTTGC10040.10746335114399233No Hit
GTAGAAACACGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT9800.10489450609672558No Hit
GTATAGTAGCGCTGAGCTTTCATGGCCTTCTGCTATTTCAAATGCTTCAT9630.10307490752157829No Hit
TTATTATTGTCATTCCTATTGCCATATCTGCTGATTCATTTACTCCAGCA9380.10039902726400877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGTCCG100.007088379145.01648145
AGTCAGT100.0070895134145.008711
GTCACGT100.0070895134145.008711
CGGTAAG100.007090648145.000963
CACGCAC2800.0134.643753
AGCACGC2950.0127.804291
GTAACAA8650.0124.885234
GCACGCA3050.0123.6073762
GAAACAC4000.0108.750734
AAACACG4000.0108.750735
TAGAAAC4250.0102.353622
TAGTAAC11250.097.956212
GAGCATC607.2759576E-1296.667316
AACACGT900.096.667316
CGCACTT3950.095.443675
ACACGTG1000.094.245587
ACGCACT4100.091.951834
CACTGTG259.386305E-487.000585
AACACGA4350.081.6672066
GAGCAGA451.2323817E-680.56041
Produced by FastQC (version 0.11.5)