FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_HBEpC_LOW_2DPI_FLUB_REP1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_HBEpC_LOW_2DPI_FLUB_REP1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362355
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA18240.13388580803094643No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT17750.1302890949862554No Hit
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT17050.1251509334938397No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT16340.1199393696943895No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT15790.11590224280749145No Hit
GTAAGAAATACAGTAAAATTGAATTTAATGCAATTCTTCTATCTCCAAAA15150.11120449515728278No Hit
ATTATTATGTGGTCATTCAATACCTCCATGTTGTCAGAGAGTACTTCCTT14900.10936943748142004No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT14550.1068003567352122No Hit
GCACTATACCTTTTCCTTTTAACTACTTTAACTGGTTTTCCTTGATAAAG14380.10555251751562553No Hit
GTTCATAAAGTTGTTGCCTTTTTGTTTCTCACCACCAAAATTAAGGAGCA14220.10437808060307335No Hit
ATACAGAAGATTGGAATATGGGACGGGGAAGAGGAGTTCCATGTAAGATG13930.10224941369907257No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT13900.10202920677796903No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC13730.10078136755838236No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAAG100.0070854006145.039291
GGGTAGT100.0070869564145.028642
GTAACAA8150.0129.008474
CACGCAC2550.0113.743773
AGCACGC2700.0104.7505951
GCACGCA2750.0102.8384862
GAAACAC4750.0100.753044
AAACACG5100.093.838615
AACACGT1400.093.229276
TAGAAAC5550.088.8463752
CGTGCCC259.3855965E-487.0044145
ACACGTG1550.084.191637
TAGTAAC13950.077.972382
GAGCAGA755.638867E-1177.354291
ACGTGCA1900.076.3308642
CGCACTT3900.074.370935
ACGCACT4200.069.058724
CACGTGC2900.065.017611
TAACAAG16850.063.2594225
AACACGA5700.062.334586
Produced by FastQC (version 0.11.5)