FastQCFastQC Report
Tue 27 Aug 2019
HNKYVAFXY_n01_HBEpC_LOW_1DPI_FLUB_REP2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHNKYVAFXY_n01_HBEpC_LOW_1DPI_FLUB_REP2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1894032
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCACGCACTTTCTTAAAATGTCGCTGTTTGGAGACACAATTGCCTACCT32960.17402029110384618No Hit
GTAGTAACAAGAGCCTTTTTTCATTTTAGTCATTTGTGTATCGCATGTAT31930.16858215700685097No Hit
GTAGTAACAAGAGCATTTTTCACTCAATTGCGTTCATTGAATTAATGGAT28300.14941669412132424No Hit
GTAGTAACAAGAGCATTTTTCAGAAACAATTAAGTTTAGTAAGGATAATT27830.14693521545570506No Hit
GTTTAGTATACCGTTAGAAAGATATAATGAAGAAACAAGGGCAAAATTGA24410.12887849835694434No Hit
ATATAACATAATAAGAAAATTCAATACATCAAGGATTGAAAAGAATCCTT24170.12761136031492606No Hit
GTAGTAACAAGAGCATTTTTCAATAACGTTTCTTTGTAATGGCAACAAGC23660.12491869197563715No Hit
CTGTAGAACATGCTTGTAAATTCAAACATTCCAGTCTCATTACAGTAACT23420.12365155393361886No Hit
GTGTAAACACAATAAGTAATGAGGAAACGAGTAACGAGCTACAGAAAACC21370.11282808315804589No Hit
TCATAGATGTGCCCGTACAGGCAGCAATTTCAACAACATTCCCATACACT21270.11230010897387162No Hit
CTACTATACTGTCTCATGGTCATGTACCTGAATCCTGGAAATTATTCAAT20790.10976583288983502No Hit
GTGTAGCATTTCTATGAACTCAGCTGATGTTGCTCCTGCCACTGGCAAGA20770.10966023805300015No Hit
CATATGGACCAATAGGAGATACTGAAGGTTTCGAAAGGGTCTACGAAAGC20760.10960744063458272No Hit
GTATAGCACCGGTCCTGTTCTCCAATAAGATAGCAAGATTGGGGAAAGGG20390.10765393615313784No Hit
ATACTAAACAGATCAGGACAAGGTATTTGAGCCTTCAGTCTTTTTGTTTT19190.10131824594304638No Hit
ATATTACTCTTAGTGCTTTTCCAAAGGATCCTTTTACTAGTTTTCCTTCT18970.10015670273786294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACAA16450.0127.817494
CACGCAC5750.0117.266313
AGCACGC5850.0116.519591
GCACGCA5850.0116.504222
GAAACAC8400.0108.755064
TTGCGAC203.865882E-4108.755053
TAGAAAC9200.0104.0293052
AAACACG8850.0103.2251365
CGCACTT6900.0102.97585
ACGCACT7350.092.7253954
TAGTAAC22450.092.367532
CTCCGAC259.388291E-486.99944145
GCCAAGC353.4381814E-582.85661145
TTTCCCG651.5661499E-978.07642145
ACGTGCA3750.075.4054952
AACACGA10100.073.2212146
ACAGCGT406.66319E-572.5033654
CCGCACT406.66319E-572.5033654
AACACGT2500.072.5033656
TAACAAG29950.070.203595
Produced by FastQC (version 0.11.5)